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Modulation of bacterial outer membrane vesicle production by envelope structure and content

Overview of attention for article published in BMC Microbiology, December 2014
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  • High Attention Score compared to outputs of the same age and source (84th percentile)

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2 X users
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1 patent

Citations

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Title
Modulation of bacterial outer membrane vesicle production by envelope structure and content
Published in
BMC Microbiology, December 2014
DOI 10.1186/s12866-014-0324-1
Pubmed ID
Authors

Carmen Schwechheimer, Adam Kulp, Meta J Kuehn

Abstract

BackgroundVesiculation is a ubiquitous secretion process of Gram-negative bacteria, where outer membrane vesicles (OMVs) are small spherical particles on the order of 50 to 250 nm composed of outer membrane (OM) and lumenal periplasmic content. Vesicle functions have been elucidated in some detail, showing their importance in virulence factor secretion, bacterial survival, and biofilm formation in pathogenesis. Furthermore, OMVs serve as an envelope stress response, protecting the secreting bacteria from internal protein misfolding stress, as well as external envelope stressors. Despite their important functional roles very little is known about the regulation and mechanism of vesicle production. Based on the envelope architecture and prior characterization of the hypervesiculation phenotypes for mutants lacking the lipoprotein, Lpp, which is involved in the covalent OM-peptidoglycan (PG) crosslinks, it is expected that an inverse relationship exists between OMV production and PG-crosslinked Lpp.ResultsIn this study, we found that subtle modifications of PG remodeling and crosslinking modulate OMV production, inversely correlating with bound Lpp levels. However, this inverse relationship was not found in strains in which OMV production is driven by an increase in ¿periplasmic pressure¿ resulting from the accumulation of protein, PG fragments, or lipopolysaccharide. In addition, the characterization of an nlpA deletion in backgrounds lacking either Lpp- or OmpA-mediated envelope crosslinks demonstrated a novel role for NlpA in envelope architecture.ConclusionsFrom this work, we conclude that OMV production can be driven by distinct Lpp concentration-dependent and Lpp concentration-independent pathways.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 231 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Israel 1 <1%
France 1 <1%
Ireland 1 <1%
Taiwan 1 <1%
Unknown 227 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 57 25%
Student > Master 40 17%
Researcher 29 13%
Student > Bachelor 23 10%
Student > Doctoral Student 15 6%
Other 13 6%
Unknown 54 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 75 32%
Agricultural and Biological Sciences 53 23%
Immunology and Microbiology 20 9%
Pharmacology, Toxicology and Pharmaceutical Science 6 3%
Medicine and Dentistry 5 2%
Other 15 6%
Unknown 57 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 May 2017.
All research outputs
#6,276,331
of 22,775,504 outputs
Outputs from BMC Microbiology
#688
of 3,184 outputs
Outputs of similar age
#85,410
of 353,115 outputs
Outputs of similar age from BMC Microbiology
#10
of 64 outputs
Altmetric has tracked 22,775,504 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 3,184 research outputs from this source. They receive a mean Attention Score of 4.1. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,115 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 64 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.