Title |
The association between Histone 3 Lysine 27 Trimethylation (H3K27me3) and prostate cancer: relationship with clinicopathological parameters
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Published in |
BMC Cancer, December 2014
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DOI | 10.1186/1471-2407-14-994 |
Pubmed ID | |
Authors |
Marjolaine Ngollo, Andre Lebert, Aslihan Dagdemir, Gaelle Judes, Seher Karsli-Ceppioglu, Marine Daures, Jean-Louis Kemeny, Frederique Penault-Llorca, Jean-Paul Boiteux, Yves-Jean Bignon, Laurent Guy, Dominique Bernard-Gallon |
Abstract |
It is well established that genetic and epigenetic alterations are common events in prostate cancer, which may lead to aberrant expression of critical genes. The importance of epigenetic mechanisms in prostate cancer carcinogenesis is increasingly evident. In this study, the focus will be on histone modifications and the primary objectives are to map H3K27me3 marks and quantify RAR beta 2, ER alpha, SRC3, RGMA, PGR, and EZH2 gene expressions in prostate cancer tissues compared to normal tissues. In addition, a data analysis was made in connection with the clinicopathological parameters.Patients and methods: 71 normal specimens and 66 cancer prostate tissues were randomly selected in order to assess the proportion of the repressive H3K27me3 chromatin marks and gene expression. H3K27me3 level was evaluated by ChIP-qPCR and mRNA expression using RT-qPCR between prostate cancer and normal tissues. Subsequently, western-blotting was performed for protein detection. The analysis of variance (ANOVA) was performed, and Tukey's test was used to correct for multiple comparisons (p-value threshold of 0.05). The principal component analysis (PCA) and discriminant factorial analysis (DFA) were used to explore association between H3K27me3 level and clinicopathological parameters. |
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Student > Master | 3 | 19% |
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Professor | 1 | 6% |
Other | 2 | 13% |
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Computer Science | 1 | 6% |
Other | 0 | 0% |
Unknown | 6 | 38% |