Title |
Comparative metagenomics of Daphnia symbionts
|
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Published in |
BMC Genomics, April 2009
|
DOI | 10.1186/1471-2164-10-172 |
Pubmed ID | |
Authors |
Weihong Qi, Guang Nong, James F Preston, Frida Ben-Ami, Dieter Ebert |
Abstract |
Shotgun sequences of DNA extracts from whole organisms allow a comprehensive assessment of possible symbionts. The current project makes use of four shotgun datasets from three species of the planktonic freshwater crustaceans Daphnia: one dataset from clones of D. pulex and D. pulicaria and two datasets from one clone of D. magna. We analyzed these datasets with three aims: First, we search for bacterial symbionts, which are present in all three species. Second, we search for evidence for Cyanobacteria and plastids, which had been suggested to occur as symbionts in a related Daphnia species. Third, we compare the metacommunities revealed by two different 454 pyrosequencing methods (GS 20 and GS FLX). |
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