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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Selection of a suitable reference gene for quantitative gene expression in mouse lymph nodes after vaccination
|
|---|---|
| Published in |
BMC Research Notes, December 2017
|
| DOI | 10.1186/s13104-017-3005-y |
| Pubmed ID | |
| Authors |
Yung-Yi C. Mosley, Harm HogenEsch |
| Abstract |
The quantification of gene expression is an important tool in the evaluation of the immune response to vaccines. Reliable reference genes for gene expression studies in mouse draining lymph nodes after vaccination have not been reported. The utility of seven potential reference genes was investigated using commercially available Taq-man primer/probe mixes. Results were evaluated with RefFinder, a web-based program including multiple algorithm methods such as geNorm, NormFinder, BestKeeper and the comparative delta-Ct. Further assessment was done by applying the candidate reference genes in relative expression calculations with genes related to the magnitude and longevity of the humoral immune responses. The ubiquitin C gene, Ubc, was found to be the most reliable reference gene when validated with well-known genes that are expressed at relatively low levels after vaccination. The optimal time of sample collection varied depending on the function of the target genes. This study identified Ubc as the most reliable reference gene and provides useful information for studies examining immunological gene expression in the draining lymph nodes after vaccination in mice. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 3 | 18% |
| Student > Master | 3 | 18% |
| Student > Ph. D. Student | 3 | 18% |
| Student > Doctoral Student | 2 | 12% |
| Student > Postgraduate | 2 | 12% |
| Other | 2 | 12% |
| Unknown | 2 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 29% |
| Biochemistry, Genetics and Molecular Biology | 5 | 29% |
| Immunology and Microbiology | 2 | 12% |
| Veterinary Science and Veterinary Medicine | 1 | 6% |
| Medicine and Dentistry | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 3 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 December 2017.
All research outputs
#17,922,331
of 23,011,300 outputs
Outputs from BMC Research Notes
#2,849
of 4,283 outputs
Outputs of similar age
#307,294
of 439,989 outputs
Outputs of similar age from BMC Research Notes
#114
of 194 outputs
Altmetric has tracked 23,011,300 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,283 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.6. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 439,989 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 194 others from the same source and published within six weeks on either side of this one. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.