Title |
Nanobodies targeting conserved epitopes on the major outer membrane protein of Campylobacter as potential tools for control of Campylobacter colonization
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Published in |
Veterinary Research, December 2017
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DOI | 10.1186/s13567-017-0491-9 |
Pubmed ID | |
Authors |
Charlotte Vanmarsenille, Inés Díaz del Olmo, Jelle Elseviers, Gholamreza Hassanzadeh Ghassabeh, Kristof Moonens, Didier Vertommen, An Martel, Freddy Haesebrouck, Frank Pasmans, Jean-Pierre Hernalsteens, Henri De Greve |
Abstract |
Campylobacter infections are among the most prevalent foodborne infections in humans, resulting in a massive disease burden worldwide. Broilers have been identified as the major source of campylobacteriosis and reducing Campylobacter loads in the broiler caeca has been proposed as an effective measure to decrease the number of infections in humans. Failure of current methods to control Campylobacter in broilers stresses the urgency to develop novel mitigation measures. We obtained six nanobodies with a broad specificity, that recognize strains belonging to the two most relevant species, Campylobacter jejuni and Campylobacter coli. The target of the nanobodies was identified as the major outer membrane protein, a porin that contributes to bacterial virulence and viability. Multimerization of the nanobodies led to agglutination of C. jejuni cells, which may affect colonization in the chicken gut. These Campylobacter-specific nanobodies may be useful to develop a strategy for preserving chickens from Campylobacter colonization. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Belgium | 1 | 25% |
Spain | 1 | 25% |
Unknown | 2 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 57 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 13 | 23% |
Student > Master | 10 | 18% |
Student > Ph. D. Student | 8 | 14% |
Student > Bachelor | 5 | 9% |
Student > Postgraduate | 2 | 4% |
Other | 3 | 5% |
Unknown | 16 | 28% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 13 | 23% |
Agricultural and Biological Sciences | 7 | 12% |
Veterinary Science and Veterinary Medicine | 3 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 5% |
Engineering | 3 | 5% |
Other | 8 | 14% |
Unknown | 20 | 35% |