Title |
PCR-based specific techniques used for detecting the most important pathogens on strawberry: a systematic review
|
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Published in |
Systematic Reviews, January 2015
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DOI | 10.1186/2046-4053-4-9 |
Pubmed ID | |
Authors |
Seyed Mahyar Mirmajlessi, Marialaura Destefanis, Richard Alexander Gottsberger, Marika Mänd, Evelin Loit |
Abstract |
Strawberry diseases are a major limiting factor that severely impact plant agronomic performance. Regarding limitations of traditional techniques for detection of pathogens, researchers have developed specific DNA-based tests as sensitive and specific techniques. The aim of this review is to provide an overview of polymerase chain reaction (PCR)-based methods used for detection or quantification of the most widespread strawberry pathogens, such as Fusarium oxysporum f.sp. fragariae, Phytophthora fragariae, Colletotrichum acutatum, Verticillium dahliae, Botrytis cinerea, Macrophomina phaseolina, and Xanthomonas fragariae. An updated and detailed list of published PCR protocols is presented and discussed, aimed at facilitating access to information that could be particularly useful for diagnostic laboratories in order to develop a rapid, cost-effective, and reliable monitoring technique. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | <1% |
Mexico | 1 | <1% |
United States | 1 | <1% |
France | 1 | <1% |
Unknown | 145 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 24 | 16% |
Researcher | 22 | 15% |
Student > Ph. D. Student | 20 | 13% |
Student > Bachelor | 17 | 11% |
Student > Doctoral Student | 6 | 4% |
Other | 21 | 14% |
Unknown | 39 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 61 | 41% |
Biochemistry, Genetics and Molecular Biology | 20 | 13% |
Engineering | 4 | 3% |
Medicine and Dentistry | 3 | 2% |
Environmental Science | 3 | 2% |
Other | 12 | 8% |
Unknown | 46 | 31% |