Title |
Development and validation of an oligonucleotide microarray to characterise ectomycorrhizal fungal communities
|
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Published in |
BMC Microbiology, November 2009
|
DOI | 10.1186/1471-2180-9-241 |
Pubmed ID | |
Authors |
Marlis Reich, Annegret Kohler, Francis Martin, Marc Buée |
Abstract |
In forest ecosystems, communities of ectomycorrhizal fungi (ECM) are influenced by several biotic and abiotic factors. To understand their underlying dynamics, ECM communities have been surveyed with ribosomal DNA-based sequencing methods. However, most identification methods are both time-consuming and limited by the number of samples that can be treated in a realistic time frame. As a result of ongoing implementation, the array technique has gained throughput capacity in terms of the number of samples and the capacity for parallel identification of several species. Thus far, although phylochips (microarrays that are used to detect species) have been mostly developed to trace bacterial communities or groups of specific fungi, no phylochip has been developed to carry oligonucleotides for several ectomycorrhizal species that belong to different genera. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 48 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 13 | 27% |
Student > Ph. D. Student | 8 | 17% |
Student > Bachelor | 5 | 10% |
Student > Postgraduate | 4 | 8% |
Student > Master | 4 | 8% |
Other | 8 | 17% |
Unknown | 6 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 29 | 60% |
Environmental Science | 5 | 10% |
Biochemistry, Genetics and Molecular Biology | 5 | 10% |
Immunology and Microbiology | 1 | 2% |
Medicine and Dentistry | 1 | 2% |
Other | 1 | 2% |
Unknown | 6 | 13% |