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Primary replication and invasion of the bovine gammaherpesvirus BoHV-4 in the genital mucosae

Overview of attention for article published in Veterinary Research, November 2017
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Title
Primary replication and invasion of the bovine gammaherpesvirus BoHV-4 in the genital mucosae
Published in
Veterinary Research, November 2017
DOI 10.1186/s13567-017-0489-3
Pubmed ID
Authors

Bo Yang, Yewei Li, Osvaldo Bogado Pascottini, Jiexiong Xie, Ruifang Wei, Geert Opsomer, Hans Nauwynck

Abstract

Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus that is widespread in cattle. Ex vivo models with bovine genital tract mucosa explants were set up to study molecular/cellular BoHV-4-host interactions. Bovine posterior vagina, cervix and uterus body were collected from cows at two stages of the reproductive cycle for making mucosa explants. The BoHV-4 replication kinetics and characteristics within the three different mucosae of animals in the follicular and luteal phase were assessed by virus titration. The number of plaques, plaque latitude and number of infected cells were determined by immunofluorescence. BoHV-4 replicated in a productive way in all genital mucosal tissues. It infected single individual cells in both epithelium and lamina propria of the genital mucosae at 24 hours post-inoculation (hpi). Later, small BoHV-4 epithelial plaques were formed that did not spread through the basement membrane. 50% of the number of BoHV-4 infected cells were identified as cytokeratin+ and CD172a+ cells in the three parts of the genital tract at 24 hpi. Upon a direct injection of genital explants with BoHV-4, fibrocytes became infected, indicating that the unidentified 50% of the infected cells are most probably fibrocytes. In this study, in vivo-related in vitro genital tract models were successfully established and the early stage of the pathogenesis of a genital infection was clarified: BoHV-4 starts with a productive infection of epithelial cells in the reproductive tract, forming small foci followed by a non-productive infection of surveilling monocytic cells which help BoHV-4 to invade into deeper tissues.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Other 3 16%
Student > Ph. D. Student 3 16%
Researcher 3 16%
Student > Master 2 11%
Student > Bachelor 2 11%
Other 2 11%
Unknown 4 21%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 6 32%
Agricultural and Biological Sciences 6 32%
Arts and Humanities 1 5%
Economics, Econometrics and Finance 1 5%
Unknown 5 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 May 2018.
All research outputs
#22,764,772
of 25,382,440 outputs
Outputs from Veterinary Research
#1,199
of 1,337 outputs
Outputs of similar age
#385,786
of 446,708 outputs
Outputs of similar age from Veterinary Research
#22
of 23 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,337 research outputs from this source. They receive a mean Attention Score of 5.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.