Title |
γδT cells but not αβT cells contribute to sepsis-induced white matter injury and motor abnormalities in mice
|
---|---|
Published in |
Journal of Neuroinflammation, December 2017
|
DOI | 10.1186/s12974-017-1029-9 |
Pubmed ID | |
Authors |
Xiaoli Zhang, Eridan Rocha-Ferreira, Tao Li, Regina Vontell, Darakhshan Jabin, Sha Hua, Kai Zhou, Arshed Nazmi, Anna-Maj Albertsson, Kristina Sobotka, Joakim Ek, Claire Thornton, Henrik Hagberg, Carina Mallard, Jianmei W. Leavenworth, Changlian Zhu, Xiaoyang Wang |
Abstract |
Infection and sepsis are associated with brain white matter injury in preterm infants and the subsequent development of cerebral palsy. In the present study, we used a neonatal mouse sepsis-induced white matter injury model to determine the contribution of different T cell subsets (αβT cells and γδT cells) to white matter injury and consequent behavioral changes. C57BL/6J wild-type (WT), T cell receptor (TCR) δ-deficient (Tcrd -/-, lacking γδT cells), and TCRα-deficient (Tcra -/-, lacking αβT cells) mice were administered with lipopolysaccharide (LPS) at postnatal day (PND) 2. Brain myelination was examined at PNDs 12, 26, and 60. Motor function and anxiety-like behavior were evaluated at PND 26 or 30 using DigiGait analysis and an elevated plus maze. White matter development was normal in Tcrd -/- and Tcrα -/- compared to WT mice. LPS exposure induced reductions in white matter tissue volume in WT and Tcrα -/- mice, but not in the Tcrd -/- mice, compared with the saline-treated groups. Neither LPS administration nor the T cell deficiency affected anxiety behavior in these mice as determined with the elevated plus maze. DigiGait analysis revealed motor function deficiency after LPS-induced sepsis in both WT and Tcrα -/- mice, but no such effect was observed in Tcrd -/- mice. Our results suggest that γδT cells but not αβT cells contribute to sepsis-induced white matter injury and subsequent motor function abnormalities in early life. Modulating the activity of γδT cells in the early stages of preterm white matter injury might represent a novel therapeutic strategy for the treatment of perinatal brain injury. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 56 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 18% |
Student > Bachelor | 9 | 16% |
Student > Master | 8 | 14% |
Student > Doctoral Student | 6 | 11% |
Researcher | 6 | 11% |
Other | 5 | 9% |
Unknown | 12 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 13 | 23% |
Neuroscience | 8 | 14% |
Nursing and Health Professions | 5 | 9% |
Immunology and Microbiology | 4 | 7% |
Psychology | 4 | 7% |
Other | 7 | 13% |
Unknown | 15 | 27% |