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Small RNA profiling for identification of miRNAs involved in regulation of saponins biosynthesis in Chlorophytum borivilianum

Overview of attention for article published in BMC Plant Biology, December 2017
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  • Good Attention Score compared to outputs of the same age and source (73rd percentile)

Mentioned by

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3 tweeters

Citations

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12 Dimensions

Readers on

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24 Mendeley
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1 CiteULike
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Title
Small RNA profiling for identification of miRNAs involved in regulation of saponins biosynthesis in Chlorophytum borivilianum
Published in
BMC Plant Biology, December 2017
DOI 10.1186/s12870-017-1214-0
Pubmed ID
Authors

Monika Kajal, Kashmir Singh

Abstract

MicroRNAs act as molecular regulator of cell signaling, plant growth and development, and regulate various primary and secondary plant metabolic processes. In the present study, deep sequencing of small RNAs was carried out to identify known and novel miRNAs from pharmaceutically important plant, Chlorophytum borivilianum. Total 442 known miRNAs and 5 novel miRNAs were identified from young leaf small RNA library. Experimental validation with stem loop RT-PCR confirmed the in silico identification. Based on transcriptome data of root and leaf of C. borivilianum, Oryza sativa, and Arabidopsis thaliana target gene prediction was done using psRNAtarget and mirRanda. BLAST2GO helped in localization of predicted targets and KEGG (Kyoto Encyclopedia for Genes and Genomes) pathway analysis concluded that miR9662, miR894, miR172, and miR166 might be involved in regulating saponin biosynthetic pathway. The correlation between miRNA and its target gene was further validated by RT-qPCR analysis. This study provides first elaborated glimpse of miRNA pool of C. borivilianum, which can help to understand the miRNA dependent regulation of saponin biosynthesis and to design further metabolic engineering experiment to enhance their contents in the plant.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 21%
Student > Master 3 13%
Researcher 2 8%
Student > Postgraduate 2 8%
Student > Bachelor 2 8%
Other 6 25%
Unknown 4 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 38%
Biochemistry, Genetics and Molecular Biology 3 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Unspecified 1 4%
Nursing and Health Professions 1 4%
Other 1 4%
Unknown 8 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 January 2018.
All research outputs
#7,114,004
of 12,376,209 outputs
Outputs from BMC Plant Biology
#602
of 1,539 outputs
Outputs of similar age
#168,057
of 351,749 outputs
Outputs of similar age from BMC Plant Biology
#28
of 126 outputs
Altmetric has tracked 12,376,209 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,539 research outputs from this source. They receive a mean Attention Score of 3.2. This one has gotten more attention than average, scoring higher than 57% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 351,749 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 126 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.