Title |
Immune responses associated with homologous protection conferred by commercial vaccines for control of avian pathogenic Escherichia coli in turkeys
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Published in |
Veterinary Research, January 2015
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DOI | 10.1186/s13567-014-0132-5 |
Pubmed ID | |
Authors |
Jean-Rémy Sadeyen, Zhiguang Wu, Holly Davies, Pauline M van Diemen, Anita Milicic, Roberto M La Ragione, Pete Kaiser, Mark P Stevens, Francis Dziva |
Abstract |
Avian pathogenic Escherichia coli (APEC) infections are a serious impediment to sustainable poultry production worldwide. Licensed vaccines are available, but the immunological basis of protection is ill-defined and a need exists to extend cross-serotype efficacy. Here, we analysed innate and adaptive responses induced by commercial vaccines in turkeys. Both a live-attenuated APEC O78 ΔaroA vaccine (Poulvac® E. coli) and a formalin-inactivated APEC O78 bacterin conferred significant protection against homologous intra-airsac challenge in a model of acute colibacillosis. Analysis of expression levels of signature cytokine mRNAs indicated that both vaccines induced a predominantly Th2 response in the spleen. Both vaccines resulted in increased levels of serum O78-specific IgY detected by ELISA and significant splenocyte recall responses to soluble APEC antigens at post-vaccination and post-challenge periods. Supplementing a non-adjuvanted inactivated vaccine with Th2-biasing (Titermax® Gold or aluminium hydroxide) or Th1-biasing (CASAC or CpG motifs) adjuvants, suggested that Th2-biasing adjuvants may give more protection. However, all adjuvants tested augmented humoral responses and protection relative to controls. Our data highlight the importance of both cell-mediated and antibody responses in APEC vaccine-mediated protection toward the control of a key avian endemic disease. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 55 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 12 | 22% |
Student > Master | 10 | 18% |
Researcher | 8 | 15% |
Student > Doctoral Student | 3 | 5% |
Lecturer | 3 | 5% |
Other | 7 | 13% |
Unknown | 12 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 11 | 20% |
Veterinary Science and Veterinary Medicine | 10 | 18% |
Biochemistry, Genetics and Molecular Biology | 7 | 13% |
Medicine and Dentistry | 4 | 7% |
Immunology and Microbiology | 3 | 5% |
Other | 4 | 7% |
Unknown | 16 | 29% |