Title |
Accelerated super-resolution imaging with FRET-PAINT
|
---|---|
Published in |
Molecular Brain, December 2017
|
DOI | 10.1186/s13041-017-0344-5 |
Pubmed ID | |
Authors |
Jongjin Lee, Sangjun Park, Wooyoung Kang, Sungchul Hohng |
Abstract |
Super-resolution fluorescence microscopy in the current form is hard to be used to image the neural connectivity of thick tissue samples due to problems such as slow imaging speed, severe photobleaching of fluorescent probes, and high background noise. Recently developed DNA-PAINT solved the photobleaching problem, but its imaging speed is extremely low. We report accelerated super-resolution fluorescence microscopy named FRET-PAINT. Compared to conventional DNA-PAINT, the imaging speed of the microscopy increases up to ~30-fold. As demonstrations, we show that 25-50 second imaging time is long enough to provide super-resolution reconstruction of microtubules and mitochondria of COS-7 cells. |
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Geographical breakdown
Country | Count | As % |
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United Kingdom | 3 | 23% |
United States | 3 | 23% |
France | 2 | 15% |
Korea, Republic of | 1 | 8% |
Unknown | 4 | 31% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 11 | 85% |
Scientists | 1 | 8% |
Science communicators (journalists, bloggers, editors) | 1 | 8% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 109 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 25 | 23% |
Student > Master | 17 | 16% |
Researcher | 16 | 15% |
Student > Bachelor | 7 | 6% |
Student > Doctoral Student | 5 | 5% |
Other | 10 | 9% |
Unknown | 29 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 23 | 21% |
Physics and Astronomy | 17 | 16% |
Chemistry | 14 | 13% |
Agricultural and Biological Sciences | 7 | 6% |
Engineering | 7 | 6% |
Other | 10 | 9% |
Unknown | 31 | 28% |