↓ Skip to main content

RNA virus interference via CRISPR/Cas13a system in plants

Overview of attention for article published in Genome Biology (Online Edition), January 2018
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
22 tweeters
patent
2 patents
facebook
1 Facebook page

Citations

dimensions_citation
589 Dimensions

Readers on

mendeley
310 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
RNA virus interference via CRISPR/Cas13a system in plants
Published in
Genome Biology (Online Edition), January 2018
DOI 10.1186/s13059-017-1381-1
Pubmed ID
Authors

Rashid Aman, Zahir Ali, Haroon Butt, Ahmed Mahas, Fatimah Aljedaani, Muhammad Zuhaib Khan, Shouwei Ding, Magdy Mahfouz

Abstract

CRISPR/Cas systems confer immunity against invading nucleic acids and phages in bacteria and archaea. CRISPR/Cas13a (known previously as C2c2) is a class 2 type VI-A ribonuclease capable of targeting and cleaving single-stranded RNA (ssRNA) molecules of the phage genome. Here, we employ CRISPR/Cas13a to engineer interference with an RNA virus, Turnip Mosaic Virus (TuMV), in plants. CRISPR/Cas13a produces interference against green fluorescent protein (GFP)-expressing TuMV in transient assays and stable overexpression lines of Nicotiana benthamiana. CRISPR RNA (crRNAs) targeting the HC-Pro and GFP sequences exhibit better interference than those targeting other regions such as coat protein (CP) sequence. Cas13a can also process pre-crRNAs into functional crRNAs. Our data indicate that CRISPR/Cas13a can be used for engineering interference against RNA viruses, providing a potential novel mechanism for RNA-guided immunity against RNA viruses and for other RNA manipulations in plants.

Twitter Demographics

The data shown below were collected from the profiles of 22 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 310 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 310 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 56 18%
Student > Ph. D. Student 47 15%
Student > Bachelor 38 12%
Student > Master 37 12%
Student > Doctoral Student 15 5%
Other 47 15%
Unknown 70 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 108 35%
Agricultural and Biological Sciences 85 27%
Immunology and Microbiology 7 2%
Chemical Engineering 5 2%
Medicine and Dentistry 4 1%
Other 19 6%
Unknown 82 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 18. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 December 2021.
All research outputs
#1,584,952
of 21,308,069 outputs
Outputs from Genome Biology (Online Edition)
#1,500
of 4,020 outputs
Outputs of similar age
#46,411
of 441,300 outputs
Outputs of similar age from Genome Biology (Online Edition)
#125
of 241 outputs
Altmetric has tracked 21,308,069 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,020 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.2. This one has gotten more attention than average, scoring higher than 62% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 441,300 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 241 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.