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Aspirin inhibited the metastasis of colon cancer cells by inhibiting the expression of toll-like receptor 4

Overview of attention for article published in Cell & Bioscience, January 2018
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1 tweeter

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Title
Aspirin inhibited the metastasis of colon cancer cells by inhibiting the expression of toll-like receptor 4
Published in
Cell & Bioscience, January 2018
DOI 10.1186/s13578-017-0198-7
Pubmed ID
Authors

Jun Ying, Hai-yang Zhou, Peng Liu, Qing You, Fei Kuang, Yi-nan Shen, Zhi-qian Hu

Abstract

The metastasis of colorectal cancer frequently tends to liver, which is one of the three leading causes of cancer-related deaths worldwide. Growing evidence showed that aspirin could effectively inhibit liver metastasis of colorectal cancer. However, the potential mechanism has not been fully understood. Mouse splenic vein metastasis assay was used to examine the metastatic ability of colon cancer cells in vivo. And wound healing and transwell assay were applied to detect the metastasis potential of C26 and HCT116 colon cancer cell lines in vitro. RT-PCR and western blotting were used to explore Toll-like receptor 4 (TLR4) expression in colon cancer cell lines. The functions of TLR4 in the migration of the colon cancer cell line were analyzed by infecting cells with lentivirus containing TLR4 siRNA. We demonstrated that lipopolysaccharides (LPS) could enhance the metastasis potential of C26 and HCT116 colon cancer cell lines. However, aspirin effectively decreased the metastasis capacity of colon cancer cells in vitro and in vivo. We found that the enhancement of LPS on the migration of colon cancer cells by inducing epithelial-mesenchymal transition (EMT) phenotype demonstrated a TLR4-dependent manner. Aspirin treatment lead to the downregulation of TLR4 on C26 cells which resulted in the decrease of C26 cells migration and EMT phenotype that induced by LPS. Additionally, the inhibitory effect from aspirin on the expression of TLR4 on C26 cells leads to the downregulation of NF-κB. The results of our study indicate that LPS origin from intestinal flora may promote the metastasis of colon cancer to liver and aspirin may inhibit the metastasis of colon cancer by inhibiting the expression of TLR4.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 27 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 26%
Student > Master 6 22%
Student > Bachelor 2 7%
Professor > Associate Professor 2 7%
Student > Doctoral Student 1 4%
Other 4 15%
Unknown 5 19%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 6 22%
Medicine and Dentistry 5 19%
Biochemistry, Genetics and Molecular Biology 3 11%
Agricultural and Biological Sciences 2 7%
Immunology and Microbiology 1 4%
Other 2 7%
Unknown 8 30%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 January 2018.
All research outputs
#11,997,118
of 13,534,507 outputs
Outputs from Cell & Bioscience
#291
of 329 outputs
Outputs of similar age
#326,838
of 386,805 outputs
Outputs of similar age from Cell & Bioscience
#15
of 26 outputs
Altmetric has tracked 13,534,507 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 329 research outputs from this source. They receive a mean Attention Score of 2.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 386,805 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 26 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.