Title |
Single nucleotide polymorphisms in genes encoding penicillin-binding proteins in β-lactamase-negative ampicillin-resistant Haemophilus influenzae in Japan
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Published in |
BMC Research Notes, January 2018
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DOI | 10.1186/s13104-018-3169-0 |
Pubmed ID | |
Authors |
Kazuhisa Misawa, Norihito Tarumoto, Shinsuke Tamura, Morichika Osa, Takaaki Hamamoto, Atsushi Yuki, Yuji Kouzaki, Kazuo Imai, Runtuwene Lucky Ronald, Toshiyuki Yamaguchi, Takashi Murakami, Shigefumi Maesaki, Yutaka Suzuki, Akihiko Kawana, Takuya Maeda |
Abstract |
β-Lactamase-negative ampicillin-resistant Haemophilus influenzae is a common opportunistic pathogen of hospital- and community-acquired infections, harboring multiple single nucleotide polymorphisms in the ftsI gene, which codes for penicillin-binding protein-3. The objectives of this study were to perform comprehensive genetic analyses of whole regions of the penicillin-binding proteins in H. influenzae and to identify additional single nucleotide polymorphisms related to antibiotic resistance, especially to ampicillin and other cephalosporins. In this genome analysis of the ftsI gene in 27 strains of H. influenzae, 10 of 23 (43.5%) specimens of group III genotype β-lactamase-negative ampicillin-resistant H. influenzae were paradoxically classified as ampicillin-sensitive phenotypes. Unfortunately, we could not identify any novel mutations that were significantly associated with ampicillin minimum inhibitory concentrations in other regions of the penicillin-binding proteins, and we reconfirmed that susceptibility to β-lactam antibiotics was mainly defined by previously reported SNPs in the ftsI gene. We should also consider detailed changes in expression that lead to antibiotic resistance in the future because the acquisition of resistance to antimicrobials can be predicted by the expression levels of a small number of genes. |
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United Kingdom | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 19 | 100% |
Demographic breakdown
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Researcher | 3 | 16% |
Student > Ph. D. Student | 3 | 16% |
Other | 1 | 5% |
Lecturer | 1 | 5% |
Student > Doctoral Student | 1 | 5% |
Other | 2 | 11% |
Unknown | 8 | 42% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 3 | 16% |
Medicine and Dentistry | 2 | 11% |
Immunology and Microbiology | 2 | 11% |
Environmental Science | 1 | 5% |
Other | 2 | 11% |
Unknown | 6 | 32% |