Title |
Study of Coxsackie B viruses interactions with Coxsackie Adenovirus receptor and Decay-Accelerating Factor using Human CaCo-2 cell line
|
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Published in |
Journal of Biomedical Science, May 2014
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DOI | 10.1186/1423-0127-21-50 |
Pubmed ID | |
Authors |
Samira Riabi, Rafik Harrath, Imed Gaaloul, Lamjed Bouslama, Dorsaf Nasri, Mahjoub Aouni, Sylvie Pillet, Bruno Pozzetto |
Abstract |
Decay Accelerating Factor (DAF) and Coxsackievirus-Adenovirus Receptor (CAR) have been identified as cellular receptors for Coxsackie B viruses (CV-B). The aim of this study is to elucidate the different binding properties of CV-B serotypes and to find out if there are any amino acid changes that could be associated to the different phenotypes.Twenty clinical CV-B isolates were tested on CaCo-2 cell line using anti-DAF (BRIC216) and anti-CAR (RmcB) antibodies. CV-B3 Nancy prototype strain and a recombinant strain (Rec, CV-B3/B4) were tested in parallel. The P1 genomic region of 12 CV-B isolates from different serotypes was sequenced and the Trans-Epithelial Electrical Resistance (TEER) along with the virus growth cycle was measured. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 30 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 17% |
Student > Master | 5 | 17% |
Student > Bachelor | 4 | 13% |
Student > Ph. D. Student | 4 | 13% |
Other | 1 | 3% |
Other | 0 | 0% |
Unknown | 11 | 37% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 23% |
Biochemistry, Genetics and Molecular Biology | 6 | 20% |
Immunology and Microbiology | 4 | 13% |
Psychology | 1 | 3% |
Sports and Recreations | 1 | 3% |
Other | 1 | 3% |
Unknown | 10 | 33% |