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A new reverse transcription-polymerase chain reaction method for accurate quantification

Overview of attention for article published in BMC Biotechnology, December 2003
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Mentioned by

wikipedia
1 Wikipedia page

Citations

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18 Dimensions

Readers on

mendeley
147 Mendeley
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Title
A new reverse transcription-polymerase chain reaction method for accurate quantification
Published in
BMC Biotechnology, December 2003
DOI 10.1186/1472-6750-3-22
Pubmed ID
Authors

Yih-Horng Shiao

Abstract

Reverse transcription-polymerase chain reaction (RT-PCR) is a very sensitive technique to measure and to compare mRNA levels among samples. However, it is extremely difficult to maintain linearity across the entire procedure, especially at the step of PCR amplification. Specific genes have been used as baseline controls to be co-amplified with target genes to normalize the amplification efficiency, but development or selection of reliable controls itself has created a new challenge.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 147 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 2 1%
Chile 2 1%
Mexico 2 1%
Vietnam 1 <1%
Brazil 1 <1%
United Kingdom 1 <1%
Germany 1 <1%
Ukraine 1 <1%
Thailand 1 <1%
Other 0 0%
Unknown 135 92%

Demographic breakdown

Readers by professional status Count As %
Student > Master 27 18%
Student > Bachelor 27 18%
Researcher 26 18%
Student > Ph. D. Student 25 17%
Student > Doctoral Student 6 4%
Other 19 13%
Unknown 17 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 64 44%
Biochemistry, Genetics and Molecular Biology 20 14%
Medicine and Dentistry 19 13%
Chemistry 9 6%
Pharmacology, Toxicology and Pharmaceutical Science 4 3%
Other 9 6%
Unknown 22 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 November 2012.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from BMC Biotechnology
#460
of 982 outputs
Outputs of similar age
#36,226
of 142,967 outputs
Outputs of similar age from BMC Biotechnology
#2
of 4 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 982 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.8. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 142,967 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.