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Altering bioelectricity on inhibition of human breast cancer cells

Overview of attention for article published in Cancer Cell International, September 2016
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  • Good Attention Score compared to outputs of the same age and source (70th percentile)

Mentioned by

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2 tweeters

Citations

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20 Dimensions

Readers on

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42 Mendeley
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Title
Altering bioelectricity on inhibition of human breast cancer cells
Published in
Cancer Cell International, September 2016
DOI 10.1186/s12935-016-0348-8
Pubmed ID
Authors

Seher Berzingi, Mackenzie Newman, Han-Gang Yu

Abstract

Membrane depolarization is associated with breast cancer. Depolarization-activated voltage-gated ion channels are directly implicated in the initiation, proliferation, and metastasis of breast cancer. In this study, the role of voltage-gated potassium and calcium ion channel modulation was explored in two different invasive ductal human carcinoma cell lines, MDA-MB-231 (triple-negative) and MCF7 (estrogen-receptor-positive). Resting membrane potential is more depolarized in MCF7 and MDA-MB-231 cells compared to normal human mammary epithelial cells. Increasing extracellular potassium concentration up to 50 mM depolarized membrane potential and greatly increased cell growth. Tetraethylammonium (TEA), a non-specific blocker of voltage-gated potassium channels, stimulated growth of MCF7 cells (control group grew by 201 %, 1 mM TEA group grew 376 %). Depolarization-induced calcium influx was hypothesized as a requirement for growth of human breast cancer. Removing calcium from culture medium stopped growth of MDA and MCF7 cells, leading to cell death after 1 week. Verapamil, a blocker of voltage-gated calcium channels clinically used in treating hypertension and coronary disease, inhibited growth of MDA cells at low concentration (10-20 μM) by 73 and 92 % after 1 and 2 days, respectively. At high concentration (100 μM), verapamil killed >90 % of MDA and MCF7 cells after 1 day. Immunoblotting experiments demonstrated that an increased expression of caspase-3, critical in apoptosis signaling, positively correlated with verapamil concentration in MDA cells. In MCF7, caspase-9 expression is increased in response to verapamil. Our results support our hypotheses that membrane depolarization and depolarization-induced calcium influx stimulate proliferation of human breast cancer cells, independently of cancer subtypes. The underlying mechanism of verapamil-induced cell death involves different caspases in MCF7 and MDA-MB-231. These data suggest that voltage-gated potassium and calcium channels may be putative targets for pharmaceutical remediation in human invasive ductal carcinomas.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
Poland 1 2%
Unknown 40 95%

Demographic breakdown

Readers by professional status Count As %
Student > Master 13 31%
Student > Bachelor 5 12%
Student > Ph. D. Student 5 12%
Researcher 4 10%
Student > Doctoral Student 3 7%
Other 6 14%
Unknown 6 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 24%
Agricultural and Biological Sciences 7 17%
Engineering 6 14%
Chemistry 4 10%
Medicine and Dentistry 4 10%
Other 4 10%
Unknown 7 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 January 2018.
All research outputs
#7,419,671
of 12,433,783 outputs
Outputs from Cancer Cell International
#138
of 468 outputs
Outputs of similar age
#176,582
of 341,616 outputs
Outputs of similar age from Cancer Cell International
#2
of 10 outputs
Altmetric has tracked 12,433,783 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 468 research outputs from this source. They receive a mean Attention Score of 2.9. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 341,616 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than 8 of them.