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Functional characterization of oligopeptide transporter 1 of dairy cows

Overview of attention for article published in Journal of Animal Science and Biotechnology, January 2018
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Title
Functional characterization of oligopeptide transporter 1 of dairy cows
Published in
Journal of Animal Science and Biotechnology, January 2018
DOI 10.1186/s40104-017-0219-8
Pubmed ID
Authors

Qingbiao Xu, Zhixuan Liu, Hongyun Liu, Fengqi Zhao, Xinbei Huang, Yueming Wu, Jianxin Liu

Abstract

It is well known that peptides play a vital role in the nutrition and health of dairy cows. Bovine oligopeptide transporter 1 (bPepT1) is involved in the peptide transport process in the gastrointestinal tracts of dairy cows. However, little information is known in the characteristics of bPepT1. Therefore, the purpose of this study was to characterize bPepT1 functionally using a mammalian cell expression system. The uptake of radiolabeled dipeptide glycyl-sarcosine ([3H]-Gly-Sar) into the bPepT1-transfected Chinese hamster ovary cells was measured at various pH and substrate concentrations and with or without 15 other small peptides that contained Met or Lys. Western blot results showed that the abundance of bPepT1 protein in the jejunum and ileum are the highest in the gastrointestinal tract of dairy cows. The uptake of [3H]-Gly-Sar by bPepT1-Chinese hamster ovary cells was dependent on time, pH, and substrate concentration, with a low Km value of 0.94 ± 0.06 mmol/L and a maximum velocity of 20.80 ± 1.74 nmol/(mg protein • 5 min). Most of the di- and tripeptides were the substrates of bPepT1, based on substrate-competitive studies. However, bPepT1 has a higher affinity to the peptides with shorter chains, greater hydrophobicity, and negative or neutral charges. These results demonstrated for the first time the functional characteristics of bPepT1, and they provide a new insight and better understanding into its vital role in absorbing a wide range of peptides from the digestive tract of dairy cows.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 23%
Professor > Associate Professor 2 15%
Student > Doctoral Student 2 15%
Student > Ph. D. Student 1 8%
Unspecified 1 8%
Other 0 0%
Unknown 4 31%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 1 8%
Unspecified 1 8%
Biochemistry, Genetics and Molecular Biology 1 8%
Agricultural and Biological Sciences 1 8%
Unknown 9 69%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 July 2023.
All research outputs
#16,725,651
of 25,382,440 outputs
Outputs from Journal of Animal Science and Biotechnology
#403
of 904 outputs
Outputs of similar age
#271,306
of 450,227 outputs
Outputs of similar age from Journal of Animal Science and Biotechnology
#15
of 21 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 904 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 49th percentile – i.e., 49% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 450,227 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.