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Large scale analysis of protein conformational transitions from aqueous to non-aqueous media

Overview of attention for article published in BMC Bioinformatics, January 2018
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (75th percentile)
  • High Attention Score compared to outputs of the same age and source (83rd percentile)

Mentioned by

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10 tweeters

Citations

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4 Dimensions

Readers on

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8 Mendeley
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Title
Large scale analysis of protein conformational transitions from aqueous to non-aqueous media
Published in
BMC Bioinformatics, January 2018
DOI 10.1186/s12859-018-2044-2
Pubmed ID
Authors

Ana Julia Velez Rueda, Alexander Miguel Monzon, Sebastián M. Ardanaz, Luis E. Iglesias, Gustavo Parisi

Abstract

Biocatalysis in organic solvents is nowadays a common practice with a large potential in Biotechnology. Several studies report that proteins which are co-crystallized or soaked in organic solvents preserve their fold integrity showing almost identical arrangements when compared to their aqueous forms. However, it is well established that the catalytic activity of proteins in organic solvents is much lower than in water. In order to explain this diminished activity and to further characterize the behaviour of proteins in non-aqueous environments, we performed a large-scale analysis (1737 proteins) of the conformational diversity of proteins crystallized in aqueous and co-crystallized or soaked in non-aqueous media. Using proteins' experimentally determined conformational diversity taken from CoDNaS database, we found that proteins in non-aqueous media display much lower conformational diversity when compared to the corresponding conformers obtained in water. When conformational diversity is compared between conformers obtained in different non-aqueous media, their structural differences are larger and mostly independent of the presence of cognate ligands. We also found that conformers corresponding to non-aqueous media have larger but less flexible cavities, lower number of disordered regions and lower active-site residue mobility. Our results show that non-aqueous media conformers have specific structural features and that they do not adopt extreme conformations found in aqueous media. This makes them clearly different from their corresponding aqueous conformers.

Twitter Demographics

The data shown below were collected from the profiles of 10 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 25%
Student > Ph. D. Student 1 13%
Professor 1 13%
Student > Bachelor 1 13%
Professor > Associate Professor 1 13%
Other 1 13%
Unknown 1 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 25%
Computer Science 2 25%
Nursing and Health Professions 1 13%
Chemistry 1 13%
Unknown 2 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2018.
All research outputs
#3,427,668
of 17,444,955 outputs
Outputs from BMC Bioinformatics
#1,459
of 6,168 outputs
Outputs of similar age
#92,645
of 375,523 outputs
Outputs of similar age from BMC Bioinformatics
#4
of 18 outputs
Altmetric has tracked 17,444,955 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 6,168 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 375,523 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.