↓ Skip to main content

Use of gene-editing technology to introduce targeted modifications in pigs

Overview of attention for article published in Journal of Animal Science and Biotechnology, January 2018
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (71st percentile)

Mentioned by

twitter
3 tweeters
patent
1 patent
googleplus
1 Google+ user

Citations

dimensions_citation
37 Dimensions

Readers on

mendeley
79 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Use of gene-editing technology to introduce targeted modifications in pigs
Published in
Journal of Animal Science and Biotechnology, January 2018
DOI 10.1186/s40104-017-0228-7
Pubmed ID
Authors

Junghyun Ryu, Randall S. Prather, Kiho Lee

Abstract

Pigs are an important resource in agriculture and serve as a model for human diseases. Due to their physiological and anatomical similarities with humans, pigs can recapitulate symptoms of human diseases, making them a useful model in biomedicine. However, in the past pig models have not been widely used partially because of the difficulty in genetic modification. The lack of true embryonic stem cells in pigs forced researchers to utilize genetic modification in somatic cells and somatic cell nuclear transfer (SCNT) to generate genetically engineered (GE) pigs carrying site-specific modifications. Although possible, this approach is extremely inefficient and GE pigs born through this method often presented developmental defects associated with the cloning process. Advancement in the gene-editing systems such as Zinc-Finger Nucleases (ZFNs), Transcription activator-like effector nucleases (TALENs), and the Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated 9 (Cas9) system have dramatically increased the efficiency of producing GE pigs. These gene-editing systems, specifically engineered endonucleases, are based on inducing double-stranded breaks (DSBs) at a specific location, and then site-specific modifications can be introduced through one of the two DNA repair pathways: non-homologous end joining (NHEJ) or homology direct repair (HDR). Random insertions or deletions (indels) can be introduced through NHEJ and specific nucleotide sequences can be introduced through HDR, if donor DNA is provided. Use of these engineered endonucleases provides a higher success in genetic modifications, multiallelic modification of the genome, and an opportunity to introduce site-specific modifications during embryogenesis, thus bypassing the need of SCNT in GE pig production. This review will provide a historical prospective of GE pig production and examples of how the gene-editing system, led by engineered endonucleases, have improved GE pig production. We will also present some of our current progress related to the optimal use of CRISPR/Cas9 system during embryogenesis.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 79 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 15 19%
Student > Bachelor 12 15%
Student > Master 8 10%
Researcher 8 10%
Student > Doctoral Student 3 4%
Other 12 15%
Unknown 21 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 22 28%
Agricultural and Biological Sciences 18 23%
Veterinary Science and Veterinary Medicine 2 3%
Physics and Astronomy 2 3%
Pharmacology, Toxicology and Pharmaceutical Science 2 3%
Other 7 9%
Unknown 26 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 December 2020.
All research outputs
#5,039,491
of 19,602,522 outputs
Outputs from Journal of Animal Science and Biotechnology
#61
of 581 outputs
Outputs of similar age
#110,307
of 387,993 outputs
Outputs of similar age from Journal of Animal Science and Biotechnology
#1
of 1 outputs
Altmetric has tracked 19,602,522 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 581 research outputs from this source. They receive a mean Attention Score of 2.4. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 387,993 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them