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An advanced glioma cell invasion assay based on organotypic brain slice cultures

Overview of attention for article published in BMC Cancer, January 2018
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3 tweeters

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Title
An advanced glioma cell invasion assay based on organotypic brain slice cultures
Published in
BMC Cancer, January 2018
DOI 10.1186/s12885-018-4007-4
Pubmed ID
Authors

Tanja Eisemann, Barbara Costa, Jens Strelau, Michel Mittelbronn, Peter Angel, Heike Peterziel

Abstract

The poor prognosis for glioblastoma patients is caused by the diffuse infiltrative growth pattern of the tumor. Therefore, the molecular and cellular processes underlying cell migration continue to be a major focus of glioblastoma research. Emerging evidence supports the concept that the tumor microenvironment has a profound influence on the functional properties of tumor cells. Accordingly, substantial effort must be devoted to move from traditional two-dimensional migration assays to three-dimensional systems that more faithfully recapitulate the complex in vivo tumor microenvironment. In order to mimic the tumor microenvironment of adult gliomas, we used adult organotypic brain slices as an invasion matrix for implanted, fluorescently labeled tumor spheroids. Cell invasion was imaged by confocal or epi-fluorescence microscopy and quantified by determining the average cumulative sprout length per spheroid. The tumor microenvironment was manipulated by treatment of the slice with small molecule inhibitors or using different genetically engineered mouse models as donors. Both epi-fluorescence and confocal microscopy were applied to precisely quantify cell invasion in this ex vivo approach. Usage of a red-emitting membrane dye in addition to tissue clearing drastically improved epi-fluorescence imaging. Preparation of brain slices from of a genetically engineered mouse with a loss of a specific cell surface protein resulted in significantly impaired tumor cell invasion. Furthermore, jasplakinolide treatment of either tumor cells or brain slice significantly reduced tumor cell invasion. We present an optimized invasion assay that closely reflects in vivo invasion by the implantation of glioma cells into organotypic adult brain slice cultures with a preserved cytoarchitecture. The diversity of applications including manipulation of the tumor cells as well as the microenvironment, permits the investigation of rate limiting factors of cell migration in a reliable context. This model will be a valuable tool for the discovery of the molecular mechanisms underlying glioma cell invasion and, ultimately, the development of novel therapeutic strategies.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 98 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 98 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 30 31%
Researcher 18 18%
Student > Bachelor 10 10%
Other 6 6%
Student > Master 6 6%
Other 12 12%
Unknown 16 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 24 24%
Agricultural and Biological Sciences 14 14%
Medicine and Dentistry 13 13%
Neuroscience 13 13%
Pharmacology, Toxicology and Pharmaceutical Science 4 4%
Other 9 9%
Unknown 21 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 February 2018.
All research outputs
#9,564,313
of 12,448,635 outputs
Outputs from BMC Cancer
#2,728
of 4,610 outputs
Outputs of similar age
#225,676
of 339,089 outputs
Outputs of similar age from BMC Cancer
#2
of 4 outputs
Altmetric has tracked 12,448,635 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,610 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 339,089 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.