Title |
Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome
|
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Published in |
Proteome Science, February 2010
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DOI | 10.1186/1477-5956-8-8 |
Pubmed ID | |
Authors |
Thorsten Stellberger, Roman Häuser, Armin Baiker, Venkata R Pothineni, Jürgen Haas, Peter Uetz |
Abstract |
Yeast two-hybrid (Y2H) screens have been among the most powerful methods to detect and analyze protein-protein interactions. However, they suffer from a significant degree of false negatives, i.e. true interactions that are not detected, and to a certain degree from false positives, i.e. interactions that appear to take place only in the context of the Y2H assay. While the fraction of false positives remains difficult to estimate, the fraction of false negatives in typical Y2H screens is on the order of 70-90%. Here we present novel Y2H vectors that significantly decrease the number of false negatives and help to mitigate the false positive problem. |
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