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Anti-inflammatory activity of Wnt signaling in enteric nervous system: in vitro preliminary evidences in rat primary cultures

Overview of attention for article published in Journal of Neuroinflammation, January 2015
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Title
Anti-inflammatory activity of Wnt signaling in enteric nervous system: in vitro preliminary evidences in rat primary cultures
Published in
Journal of Neuroinflammation, January 2015
DOI 10.1186/s12974-015-0248-1
Pubmed ID
Authors

Rosa Di Liddo, Thomas Bertalot, Anne Schuster, Sandra Schrenk, Alessia Tasso, Ilenia Zanusso, Maria Conconi, Karl Schäfer

Abstract

BackgroundIn the last years, Wnt signaling was demonstrated to regulate inflammatory processes. In particular, an increased expression of Wnts and Frizzled receptors was reported in inflammatory bowel disease (IBD) and ulcerative colitis to exert both anti- and pro-inflammatory functions regulating the intestinal activated nuclear factor ¿B (NF-¿B), TNFa release, and IL10 expression.MethodsTo investigate the role of Wnt pathway in the response of the enteric nervous system (ENS) to inflammation, neurons and glial cells from rat myenteric plexus were treated with exogenous Wnt3a and/or LPS with or without supporting neurotrophic factors such as basic fibroblast growth factor (bFGF), epithelial growth factor (EGF), and glial cell-derived neurotrophic factor (GDNF). The immunophenotypical characterization by flow cytometry and the protein and gene expression analysis by qPCR and western blotting were carried out.ResultsFlow cytometry and immunofluorescence staining evidenced that enteric neurons coexpressed Frizzled 9 and toll-like receptor 4 (TLR4) while glial cells were immunoreactive to TLR4 and Wnt3a suggesting that canonical Wnt signaling is active in ENS.Under in vitro LPS treatment, Western blot analysis demonstrated an active cross talk between canonical Wnt signaling and NF-¿B pathway that is essential to negatively control enteric neuronal response to inflammatory stimuli. Upon costimulation with LPS and Wnt3a, a significant anti-inflammatory activity was detected by RT-PCR based on an increased IL10 expression and a downregulation of pro-inflammatory cytokines TNFa, IL1B, and interleukin 6 (IL6). When the availability of neurotrophic factors in ENS cultures was abolished, a changed cell reactivity by Wnt signaling was observed at basal conditions and after LPS treatment.ConclusionsThe results of this study suggested the existence of neuronal surveillance through FZD9 and Wnt3a in enteric myenteric plexus. Moreover, experimental evidences were provided to clarify the correlation among soluble trophic factors, Wnt signaling, and anti-inflammatory protection of ENS.

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Mendeley readers

The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 58 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 19%
Student > Master 9 16%
Student > Bachelor 8 14%
Student > Doctoral Student 6 10%
Researcher 5 9%
Other 11 19%
Unknown 8 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 28%
Biochemistry, Genetics and Molecular Biology 10 17%
Medicine and Dentistry 9 16%
Immunology and Microbiology 5 9%
Neuroscience 4 7%
Other 3 5%
Unknown 11 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 February 2015.
All research outputs
#3,953,479
of 4,720,494 outputs
Outputs from Journal of Neuroinflammation
#621
of 718 outputs
Outputs of similar age
#132,542
of 164,602 outputs
Outputs of similar age from Journal of Neuroinflammation
#18
of 25 outputs
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