Title |
Detection and quantification of extracellular microRNAs in murine biofluids
|
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Published in |
Biological Procedures Online, March 2014
|
DOI | 10.1186/1480-9222-16-5 |
Pubmed ID | |
Authors |
Thomas C Roberts, Anna M L Coenen-Stass, Corinne A Betts, Matthew J A Wood |
Abstract |
MicroRNAs (miRNAs) are short RNA molecules which regulate gene expression in eukaryotic cells, and are abundant and stable in biofluids such as blood serum and plasma. As such, there has been heightened interest in the utility of extracellular miRNAs as minimally invasive biomarkers for diagnosis and monitoring of a wide range of human pathologies. However, quantification of extracellular miRNAs is subject to a number of specific challenges, including the relatively low RNA content of biofluids, the possibility of contamination with serum proteins (including RNases and PCR inhibitors), hemolysis, platelet contamination/activation, a lack of well-established reference miRNAs and the biochemical properties of miRNAs themselves. Protocols for the detection and quantification of miRNAs in biofluids are therefore of high interest. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Finland | 1 | 1% |
Iraq | 1 | 1% |
Sri Lanka | 1 | 1% |
Austria | 1 | 1% |
Unknown | 91 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 21 | 22% |
Student > Master | 17 | 18% |
Student > Ph. D. Student | 16 | 17% |
Student > Bachelor | 8 | 8% |
Student > Doctoral Student | 6 | 6% |
Other | 19 | 20% |
Unknown | 8 | 8% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 26 | 27% |
Biochemistry, Genetics and Molecular Biology | 23 | 24% |
Medicine and Dentistry | 14 | 15% |
Neuroscience | 8 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 4% |
Other | 9 | 9% |
Unknown | 11 | 12% |