Title |
Maitotoxin-induced membrane blebbing and cell death in bovine aortic endothelial cells
|
---|---|
Published in |
BMC Physiology, February 2001
|
DOI | 10.1186/1472-6793-1-2 |
Pubmed ID | |
Authors |
Mark Estacion, William P Schilling |
Abstract |
Maitotoxin, a potent cytolytic agent, causes an increase in cytosolic free Ca2+ concentration ([Ca2+]i) via activation of Ca2+-permeable, non-selective cation channels (CaNSC). Channel activation is followed by formation of large endogenous pores that allow ethidium and propidium-based vital dyes to enter the cell. Although activation of these cytolytic/oncotic pores, or COP, precedes release of lactate dehydrogenase, an indication of oncotic cell death, the relationship between CaNSC, COP, membrane lysis, and the associated changes in cell morphology has not been clearly defined. In the present study, the effect maitotoxin on [Ca2+]i, vital dye uptake, lactate dehydrogenase release, and membrane blebbing was examined in bovine aortic endothelial cells. |
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