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Heterologous expression and antitumor activity analysis of syringolin from Pseudomonas syringae pv. syringae B728a

Overview of attention for article published in Microbial Cell Factories, February 2018
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Title
Heterologous expression and antitumor activity analysis of syringolin from Pseudomonas syringae pv. syringae B728a
Published in
Microbial Cell Factories, February 2018
DOI 10.1186/s12934-018-0859-1
Pubmed ID
Authors

Fan Huang, Jianli Tang, Lian He, Xuezhi Ding, Shaoya Huang, Youming Zhang, Yunjun Sun, Liqiu Xia

Abstract

Syringolin, synthesized by a mixed non-ribosomal peptide synthetase/polyketide synthetase in Pseudomonas syringae pv. syringae (Pss) B728a, is a novel eukaryotic proteasome inhibitor. Meanwhile, directly modifying large fragments in the PKS/NRPS gene cluster through traditional DNA engineering techniques is very difficult. In this study, we directly cloned the syl gene cluster from Pss B301D-R via Red/ET recombineering to effectively express syringolin in heterologous hosts. A 22 kb genomic fragment containing the sylA-sylE gene cluster was cloned into the pASK vector, and the obtained recombinant plasmid was transferred into Streptomyces coelicolor and Streptomyces lividans for the heterologous expression of syringolin. Transcriptional levels of recombinant syl gene in S. coelicolor M145 and S. lividans TK24 were evaluated via RT-PCR and the production of syringolin compounds was detected via LC-MS analysis. The extracts of the engineered bacteria showed cytotoxic activity to B16, 4T1, Meth-A, and HeLa tumor cells. It is noteworthy that the syringolin displayed anticancer activity against C57BL/6 mice with B16 murine melanoma tumor cells. Together, our results herein demonstrate the potential of syrinolin as effective antitumor agent that can treat various cancers without apparent adverse effects. This present study is the first to report the heterologous expression of the entire syl gene cluster in Streptomyces strains and the successful expression of syringolin in both S. coelicolor M145 and S. lividans TK24. Syringolin derivatives demonstrated high cytotoxicity in vitro and in vivo. Hence, this paper provided an important foundation for the discovery and production of new antitumor compounds.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 14%
Student > Ph. D. Student 3 14%
Student > Bachelor 2 10%
Researcher 2 10%
Lecturer 1 5%
Other 2 10%
Unknown 8 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 24%
Chemistry 3 14%
Agricultural and Biological Sciences 1 5%
Environmental Science 1 5%
Earth and Planetary Sciences 1 5%
Other 1 5%
Unknown 9 43%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 February 2018.
All research outputs
#14,968,843
of 23,025,074 outputs
Outputs from Microbial Cell Factories
#931
of 1,613 outputs
Outputs of similar age
#199,956
of 330,211 outputs
Outputs of similar age from Microbial Cell Factories
#24
of 40 outputs
Altmetric has tracked 23,025,074 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,613 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 330,211 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 40 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.