Title |
Optimized high gradient magnetic separation for isolation of Plasmodium-infected red blood cells
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Published in |
Malaria Journal, February 2010
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DOI | 10.1186/1475-2875-9-38 |
Pubmed ID | |
Authors |
Sebastian C Bhakdi, Annette Ottinger, Sangdao Somsri, Panudda Sratongno, Peeranad Pannadaporn, Pattamawan Chimma, Prida Malasit, Kovit Pattanapanyasat, Hartmut PH Neumann |
Abstract |
Highly purified infected red blood cells (irbc), or highly synchronized parasite cultures, are regularly required in malaria research. Conventional isolation and synchronization rely on density and osmotic fragility of irbc, respectively. High gradient magnetic separation (HGMS) offers an alternative based on intrinsic magnetic properties of irbc, avoiding exposure to chemicals and osmotic stress. Successful HGMS concentration in malaria research was previously reported using polymer coated columns, while HGMS depletion has not been described yet. This study presents a new approach to both HGMS concentration and depletion in malaria research, rendering polymer coating unnecessary. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
India | 3 | 4% |
South Africa | 1 | 1% |
United Kingdom | 1 | 1% |
Canada | 1 | 1% |
United States | 1 | 1% |
Unknown | 74 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 19 | 23% |
Researcher | 16 | 20% |
Student > Master | 7 | 9% |
Student > Bachelor | 6 | 7% |
Student > Doctoral Student | 5 | 6% |
Other | 12 | 15% |
Unknown | 16 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 25 | 31% |
Engineering | 15 | 19% |
Medicine and Dentistry | 9 | 11% |
Physics and Astronomy | 3 | 4% |
Immunology and Microbiology | 2 | 2% |
Other | 9 | 11% |
Unknown | 18 | 22% |