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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Transient transformation of Podosphaera xanthii by electroporation of conidia
|
|---|---|
| Published in |
BMC Microbiology, February 2015
|
| DOI | 10.1186/s12866-014-0338-8 |
| Pubmed ID | |
| Authors |
David Vela-Corcía, Diego Romero, Juan Antonio Torés, Antonio De Vicente, Alejandro Pérez-García |
| Abstract |
BackgroundPowdery mildew diseases are a major phytosanitary issue causing important yield and economic losses in agronomic, horticultural and ornamental crops. Powdery mildew fungi are obligate biotrophic parasites unable to grow on culture media, a fact that has significantly limited their genetic manipulation. In this work, we report a protocol based on the electroporation of fungal conidia, for the transient transformation of Podosphaera fusca (synonym Podosphaera xanthii), the main causal agent of cucurbit powdery mildew.ResultsTo introduce DNA into P. xanthii conidia, we applied two square-wave pulses of 1.7 kV for 1 ms with an interval of 5 s. We tested these conditions with several plasmids bearing as selective markers hygromycin B resistance (hph), carbendazim resistance (TUB2) or GFP (gfp) under control of endogenous regulatory elements from Aspergillus nidulans, Neurospora crassa or P. xanthii to drive their expression. An in planta selection procedure using the MBC fungicide carbendazim permitted the propagation of transformants onto zucchini cotyledons and avoided the phytotoxicity associated with hygromycin B.ConclusionThis is the first report on the transformation of P. xanthii and the transformation of powdery mildew fungi using electroporation. Although the transformants are transient, this represents a feasible method for the genetic manipulation of this important group of plant pathogens. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 4% |
| Spain | 1 | 2% |
| Unknown | 51 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 11 | 20% |
| Student > Ph. D. Student | 9 | 17% |
| Student > Master | 9 | 17% |
| Researcher | 7 | 13% |
| Student > Postgraduate | 3 | 6% |
| Other | 7 | 13% |
| Unknown | 8 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 32 | 59% |
| Biochemistry, Genetics and Molecular Biology | 9 | 17% |
| Veterinary Science and Veterinary Medicine | 1 | 2% |
| Unspecified | 1 | 2% |
| Immunology and Microbiology | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 8 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 February 2015.
All research outputs
#13,350,710
of 22,787,797 outputs
Outputs from BMC Microbiology
#1,250
of 3,187 outputs
Outputs of similar age
#172,104
of 352,111 outputs
Outputs of similar age from BMC Microbiology
#25
of 64 outputs
Altmetric has tracked 22,787,797 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,187 research outputs from this source. They receive a mean Attention Score of 4.1. This one has gotten more attention than average, scoring higher than 60% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,111 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 64 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.