Title |
Superresolution fluorescence microscopy for 3D reconstruction of thick samples
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Published in |
Molecular Brain, March 2018
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DOI | 10.1186/s13041-018-0361-z |
Pubmed ID | |
Authors |
Sangjun Park, Wooyoung Kang, Yeong-Dae Kwon, Jaehoon Shim, Siyong Kim, Bong-Kiun Kaang, Sungchul Hohng |
Abstract |
Three-dimensional (3D) reconstruction of thick samples using superresolution fluorescence microscopy remains challenging due to high level of background noise and fast photobleaching of fluorescence probes. We develop superresolution fluorescence microscopy that can reconstruct 3D structures of thick samples with both high localization accuracy and no photobleaching problem. The background noise is reduced by optically sectioning the sample using line-scan confocal microscopy, and the photobleaching problem is overcome by using the DNA-PAINT (Point Accumulation for Imaging in Nanoscale Topography). As demonstrations, we take 3D superresolution images of microtubules of a whole cell, and two-color 3D images of microtubules and mitochondria. We also present superresolution images of chemical synapse of a mouse brain section at different z-positions ranging from 0 μm to 100 μm. |
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Demographic breakdown
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Members of the public | 5 | 83% |
Science communicators (journalists, bloggers, editors) | 1 | 17% |
Mendeley readers
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Researcher | 11 | 15% |
Student > Master | 9 | 13% |
Student > Bachelor | 6 | 8% |
Professor | 3 | 4% |
Other | 3 | 4% |
Unknown | 19 | 26% |
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Agricultural and Biological Sciences | 5 | 7% |
Other | 12 | 17% |
Unknown | 19 | 26% |