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Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming

Overview of attention for article published in Stem Cell Research & Therapy, March 2018
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

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1 blog
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9 Dimensions

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27 Mendeley
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Title
Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming
Published in
Stem Cell Research & Therapy, March 2018
DOI 10.1186/s13287-018-0796-2
Pubmed ID
Authors

Srijaya Thekkeparambil Chandrabose, Sandhya Sriram, Subha Subramanian, Shanshan Cheng, Wee Kiat Ong, Steve Rozen, Noor Hayaty Abu Kasim, Shigeki Sugii

Abstract

While a shift towards non-viral and animal component-free methods of generating induced pluripotent stem (iPS) cells is preferred for safer clinical applications, there is still a shortage of reliable cell sources and protocols for efficient reprogramming. Here, we show a robust episomal and xeno-free reprogramming strategy for human iPS generation from dental pulp stem cells (DPSCs) which renders good efficiency (0.19%) over a short time frame (13-18 days). The robustness of DPSCs as starting cells for iPS induction is found due to their exceptional inherent stemness properties, developmental origin from neural crest cells, specification for tissue commitment, and differentiation capability. To investigate the epigenetic basis for the high reprogramming efficiency of DPSCs, we performed genome-wide DNA methylation analysis and found that the epigenetic signature of DPSCs associated with pluripotent, developmental, and ecto-mesenchymal genes is relatively close to that of iPS and embryonic stem (ES) cells. Among these genes, it is found that overexpression of PAX9 and knockdown of HERV-FRD improved the efficiencies of iPS generation. In conclusion, our study provides underlying epigenetic mechanisms that establish a robust platform for efficient generation of iPS cells from DPSCs, facilitating industrial and clinical use of iPS technology for therapeutic needs.

X Demographics

X Demographics

The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 27 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 11%
Student > Master 3 11%
Student > Ph. D. Student 3 11%
Lecturer > Senior Lecturer 2 7%
Professor 1 4%
Other 2 7%
Unknown 13 48%
Readers by discipline Count As %
Medicine and Dentistry 7 26%
Biochemistry, Genetics and Molecular Biology 5 19%
Nursing and Health Professions 1 4%
Agricultural and Biological Sciences 1 4%
Design 1 4%
Other 0 0%
Unknown 12 44%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 April 2018.
All research outputs
#3,006,909
of 23,577,761 outputs
Outputs from Stem Cell Research & Therapy
#239
of 2,496 outputs
Outputs of similar age
#63,803
of 333,638 outputs
Outputs of similar age from Stem Cell Research & Therapy
#7
of 70 outputs
Altmetric has tracked 23,577,761 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,496 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 333,638 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 70 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.