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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Gibson Deletion: a novel application of isothermal in vitro recombination
|
|---|---|
| Published in |
Biological Procedures Online, January 2018
|
| DOI | 10.1186/s12575-018-0068-7 |
| Pubmed ID | |
| Authors |
Swara Kalva, Jef D. Boeke, Paolo Mita |
| Abstract |
Recombinant DNA technology is today a fundamental tool for virtually all biological research fields. Among the many techniques available for the construction of a "custom DNA" molecule, the isothermal in vitro assembly, or Gibson assembly, allows for an efficient, one-step, scarless recombination-based assembly. Here, we apply and characterize the use of Gibson assembly for the deletion of DNA sequences around a DNA cut. This method, that we named "Gibson Deletion", can be used to easily substitute or delete one or more restriction sites within a DNA molecule. We show that Gibson Deletion is a viable method to delete up to 100 nucleotides from the DNA ends of a cleavage site. In addition, we found that Gibson Deletion can be performed using single strand DNA with the same efficiency as using double strand DNA molecules. Gibson Deletion is a novel, easy and convenient application of isothermal in vitro assembly, that performs with high efficiency and can be implemented for a broad range of applications. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Canada | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 63 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 18 | 29% |
| Student > Ph. D. Student | 7 | 11% |
| Student > Bachelor | 7 | 11% |
| Student > Master | 5 | 8% |
| Student > Doctoral Student | 4 | 6% |
| Other | 6 | 10% |
| Unknown | 16 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 22 | 35% |
| Agricultural and Biological Sciences | 9 | 14% |
| Medicine and Dentistry | 5 | 8% |
| Immunology and Microbiology | 3 | 5% |
| Neuroscience | 2 | 3% |
| Other | 3 | 5% |
| Unknown | 19 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 April 2022.
All research outputs
#7,359,319
of 25,382,440 outputs
Outputs from Biological Procedures Online
#59
of 192 outputs
Outputs of similar age
#140,321
of 451,056 outputs
Outputs of similar age from Biological Procedures Online
#1
of 3 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 192 research outputs from this source. They receive a mean Attention Score of 4.6. This one has gotten more attention than average, scoring higher than 68% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 451,056 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them