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Rescue and characterization of recombinant cedar virus, a non-pathogenic Henipavirus species

Overview of attention for article published in Virology Journal, March 2018
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (55th percentile)

Mentioned by

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4 tweeters

Citations

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13 Dimensions

Readers on

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32 Mendeley
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Title
Rescue and characterization of recombinant cedar virus, a non-pathogenic Henipavirus species
Published in
Virology Journal, March 2018
DOI 10.1186/s12985-018-0964-0
Pubmed ID
Authors

Eric D. Laing, Moushimi Amaya, Chanakha K. Navaratnarajah, Yan-Ru Feng, Roberto Cattaneo, Lin-Fa Wang, Christopher C. Broder

Abstract

Hendra virus and Nipah virus are zoonotic viruses that have caused severe to fatal disease in livestock and human populations. The isolation of Cedar virus, a non-pathogenic virus species in the genus Henipavirus, closely-related to the highly pathogenic Hendra virus and Nipah virus offers an opportunity to investigate differences in pathogenesis and receptor tropism among these viruses. We constructed full-length cDNA clones of Cedar virus from synthetic oligonucleotides and rescued two replication-competent, recombinant Cedar virus variants: a recombinant wild-type Cedar virus and a recombinant Cedar virus that expresses a green fluorescent protein from an open reading frame inserted between the phosphoprotein and matrix genes. Replication kinetics of both viruses and stimulation of the interferon pathway were characterized in vitro. Cellular tropism for ephrin-B type ligands was qualitatively investigated by microscopy and quantitatively by a split-luciferase fusion assay. Successful rescue of recombinant Cedar virus expressing a green fluorescent protein did not significantly affect virus replication compared to the recombinant wild-type Cedar virus. We demonstrated that recombinant Cedar virus stimulated the interferon pathway and utilized the established Hendra virus and Nipah virus receptor, ephrin-B2, but not ephrin-B3 to mediate virus entry. We further characterized virus-mediated membrane fusion kinetics of Cedar virus with the known henipavirus receptors ephrin-B2 and ephrin-B3. The recombinant Cedar virus platform may be utilized to characterize the determinants of pathogenesis across the henipaviruses, investigate their receptor tropisms, and identify novel pan-henipavirus antivirals. Moreover, these experiments can be conducted safely under BSL-2 conditions.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 22%
Student > Bachelor 6 19%
Student > Master 4 13%
Researcher 4 13%
Other 3 9%
Other 5 16%
Unknown 3 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 25%
Agricultural and Biological Sciences 7 22%
Unspecified 2 6%
Economics, Econometrics and Finance 2 6%
Veterinary Science and Veterinary Medicine 2 6%
Other 7 22%
Unknown 4 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 December 2018.
All research outputs
#8,639,345
of 16,346,525 outputs
Outputs from Virology Journal
#888
of 2,482 outputs
Outputs of similar age
#122,255
of 282,294 outputs
Outputs of similar age from Virology Journal
#1
of 1 outputs
Altmetric has tracked 16,346,525 research outputs across all sources so far. This one is in the 46th percentile – i.e., 46% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,482 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.6. This one has gotten more attention than average, scoring higher than 62% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 282,294 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them