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Sample prep for proteomics of breast cancer: proteomics and gene ontology reveal dramatic differences in protein solubilization preferences of radioimmunoprecipitation assay and urea lysis buffers

Overview of attention for article published in Proteome Science, October 2008
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About this Attention Score

  • Among the highest-scoring outputs from this source (#45 of 208)

Mentioned by

wikipedia
2 Wikipedia pages

Citations

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76 Dimensions

Readers on

mendeley
225 Mendeley
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Title
Sample prep for proteomics of breast cancer: proteomics and gene ontology reveal dramatic differences in protein solubilization preferences of radioimmunoprecipitation assay and urea lysis buffers
Published in
Proteome Science, October 2008
DOI 10.1186/1477-5956-6-30
Pubmed ID
Authors

Lambert CM Ngoka

Abstract

An important step in the proteomics of solid tumors, including breast cancer, consists of efficiently extracting most of proteins in the tumor specimen. For this purpose, Radio-Immunoprecipitation Assay (RIPA) buffer is widely employed. RIPA buffer's rapid and highly efficient cell lysis and good solubilization of a wide range of proteins is further augmented by its compatibility with protease and phosphatase inhibitors, ability to minimize non-specific protein binding leading to a lower background in immunoprecipitation, and its suitability for protein quantitation.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 225 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 1%
Germany 3 1%
Portugal 1 <1%
Bulgaria 1 <1%
Philippines 1 <1%
Unknown 216 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 53 24%
Researcher 39 17%
Student > Bachelor 30 13%
Student > Master 27 12%
Student > Doctoral Student 9 4%
Other 34 15%
Unknown 33 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 65 29%
Biochemistry, Genetics and Molecular Biology 46 20%
Medicine and Dentistry 21 9%
Chemistry 11 5%
Engineering 10 4%
Other 34 15%
Unknown 38 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 May 2017.
All research outputs
#8,534,528
of 25,371,288 outputs
Outputs from Proteome Science
#45
of 208 outputs
Outputs of similar age
#37,075
of 103,638 outputs
Outputs of similar age from Proteome Science
#1
of 4 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 208 research outputs from this source. They receive a mean Attention Score of 2.8. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 103,638 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them