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BS-Seeker3: ultrafast pipeline for bisulfite sequencing

Overview of attention for article published in BMC Bioinformatics, April 2018
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  • Good Attention Score compared to outputs of the same age (65th percentile)
  • Good Attention Score compared to outputs of the same age and source (69th percentile)

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11 X users

Citations

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87 Mendeley
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Title
BS-Seeker3: ultrafast pipeline for bisulfite sequencing
Published in
BMC Bioinformatics, April 2018
DOI 10.1186/s12859-018-2120-7
Pubmed ID
Authors

Kevin Yu Yuan Huang, Yan-Jiun Huang, Pao-Yang Chen

Abstract

DNA methylation is an important epigenetic modification critical in regulation and transgenerational inheritance. The methylation level can be estimated at single-nucleotide resolution by whole-genome bisulfite sequencing (BS-seq; WGBS). Current bisulfite aligners provide pipelines for processing the reads by WGBS; however, few are able to analyze the BS-seqs in a reasonable timeframe that meets the needs of the rapid expansion of epigenome sequencing in biomedical research. We introduce BS-Seeker3, an extensively improved and optimized implementation of BS-Seeker2 that leverages the available computational power of a standard bioinformatics lab. BS-Seeker3 adopts all alignment features of BS-Seeker2. It performs ultrafast alignments and achieves both high accuracy and high mappability, more than twice that of the other aligners that we evaluated. Moreover, BS Seeker 3 is well linked with downstream analyzer MethGo for up to 9 types of genomic and epigenomic analyses. BS-Seeker3 is an accurate, versatile, ultra-fast pipeline for processing bisulfite-converted reads. It also helps the user better visualize the methylation data.

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X Demographics

The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 87 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 87 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 24 28%
Student > Ph. D. Student 20 23%
Student > Master 9 10%
Student > Bachelor 8 9%
Student > Doctoral Student 3 3%
Other 6 7%
Unknown 17 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 27 31%
Agricultural and Biological Sciences 23 26%
Computer Science 6 7%
Medicine and Dentistry 6 7%
Neuroscience 2 2%
Other 7 8%
Unknown 16 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 January 2020.
All research outputs
#6,535,395
of 23,577,654 outputs
Outputs from BMC Bioinformatics
#2,455
of 7,400 outputs
Outputs of similar age
#113,032
of 330,441 outputs
Outputs of similar age from BMC Bioinformatics
#33
of 109 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 7,400 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one has gotten more attention than average, scoring higher than 66% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 330,441 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 109 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.