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Genetic variation associated with increased insecticide resistance in the malaria mosquito, Anopheles coluzzii

Overview of attention for article published in Parasites & Vectors, April 2018
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  • Good Attention Score compared to outputs of the same age (65th percentile)

Mentioned by

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6 tweeters

Citations

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17 Dimensions

Readers on

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74 Mendeley
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Title
Genetic variation associated with increased insecticide resistance in the malaria mosquito, Anopheles coluzzii
Published in
Parasites & Vectors, April 2018
DOI 10.1186/s13071-018-2817-5
Pubmed ID
Authors

Bradley J. Main, Amanda Everitt, Anthony J. Cornel, Fereydoun Hormozdiari, Gregory C. Lanzaro

Abstract

Malaria mortality rates in sub-Saharan Africa have declined significantly in recent years as a result of increased insecticide-treated bed net (ITN) usage. A major challenge to further progress is the emergence and spread of insecticide resistance alleles in the Anopheles mosquito vectors, like An. coluzzii. A non-synonymous mutation in the para voltage-gated sodium channel gene reduces pyrethroid-binding affinity, resulting in knockdown resistance (kdr). Metabolic mechanisms of insecticide resistance involving detoxification genes like cytochrome P450 genes, carboxylesterases, and glutathione S-transferases are also important. As some gene activity is tissue-specific and/or environmentally induced, gene regulatory variation may be overlooked when comparing expression from whole mosquito bodies under standard rearing conditions. We detected complex insecticide resistance in a 2014 An. coluzzii colony from southern Mali using bottle bioassays. Additional bioassays involving recombinant genotypes from a cross with a relatively susceptible 1995 An. coluzzii colony from Mali confirmed the importance of kdr and associated increased permethrin resistance to the CYP9K1 locus on the X chromosome. Significant differential expression of CYP9K1 was not observed among these colonies in Malpighian tubules. However, the P450 gene CYP6Z1 was overexpressed in resistant individuals following sublethal permethrin exposure and the carboxylesterase gene COEAE5G was constitutively overexpressed. The significant P450-related insecticide resistance observed in the 2014 An. coluzzii colony indicates that ITNs treated with the P450 inhibitor piperonyl butoxide (PBO) would be more effective in this region. The known insecticide resistance gene CYP6Z1 was differentially expressed exclusively in the context of sublethal permethrin exposure, highlighting the importance of tissue-specificity and environmental conditions in gene expression studies. The increased activity of the carboxylesterase COEAE5G in the resistant An. coluzzii colony suggests resistance to other insecticides like organophosphates. Additional gene expression studies involving other tissues (e.g. fat body) would provide a more comprehensive view of genes underlying metabolic insecticide resistance in An. coluzzii from Mali. Identifying genetic markers linked to these regulatory alleles is an important next step that would substantially improve insecticide resistance surveillance and population genetic studies in this important vector species.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 74 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 19%
Researcher 10 14%
Student > Master 9 12%
Student > Bachelor 8 11%
Student > Doctoral Student 7 9%
Other 9 12%
Unknown 17 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 19 26%
Biochemistry, Genetics and Molecular Biology 18 24%
Immunology and Microbiology 4 5%
Medicine and Dentistry 4 5%
Environmental Science 3 4%
Other 10 14%
Unknown 16 22%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 April 2018.
All research outputs
#4,556,293
of 16,005,717 outputs
Outputs from Parasites & Vectors
#1,034
of 4,290 outputs
Outputs of similar age
#96,710
of 281,535 outputs
Outputs of similar age from Parasites & Vectors
#1
of 2 outputs
Altmetric has tracked 16,005,717 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 4,290 research outputs from this source. They receive a mean Attention Score of 4.8. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 281,535 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them