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High quality copy number and genotype data from FFPE samples using Molecular Inversion Probe (MIP) microarrays

Overview of attention for article published in BMC Medical Genomics, February 2009
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • Good Attention Score compared to outputs of the same age and source (71st percentile)

Mentioned by

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3 patents
wikipedia
1 Wikipedia page

Citations

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81 Dimensions

Readers on

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72 Mendeley
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1 CiteULike
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Title
High quality copy number and genotype data from FFPE samples using Molecular Inversion Probe (MIP) microarrays
Published in
BMC Medical Genomics, February 2009
DOI 10.1186/1755-8794-2-8
Pubmed ID
Authors

Yuker Wang, Victoria EH Carlton, George Karlin-Neumann, Ronald Sapolsky, Li Zhang, Martin Moorhead, Zhigang C Wang, Andrea L Richardson, Robert Warren, Axel Walther, Melissa Bondy, Aysegul Sahin, Ralf Krahe, Musaffe Tuna, Patricia A Thompson, Paul T Spellman, Joe W Gray, Gordon B Mills, Malek Faham

Abstract

A major challenge facing DNA copy number (CN) studies of tumors is that most banked samples with extensive clinical follow-up information are Formalin-Fixed Paraffin Embedded (FFPE). DNA from FFPE samples generally underperforms or suffers high failure rates compared to fresh frozen samples because of DNA degradation and cross-linking during FFPE fixation and processing. As FFPE protocols may vary widely between labs and samples may be stored for decades at room temperature, an ideal FFPE CN technology should work on diverse sample sets. Molecular Inversion Probe (MIP) technology has been applied successfully to obtain high quality CN and genotype data from cell line and frozen tumor DNA. Since the MIP probes require only a small (approximately 40 bp) target binding site, we reasoned they may be well suited to assess degraded FFPE DNA. We assessed CN with a MIP panel of 50,000 markers in 93 FFPE tumor samples from 7 diverse collections. For 38 FFPE samples from three collections we were also able to asses CN in matched fresh frozen tumor tissue.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 3 4%
United States 2 3%
Germany 1 1%
France 1 1%
Netherlands 1 1%
Belgium 1 1%
Canada 1 1%
Unknown 62 86%

Demographic breakdown

Readers by professional status Count As %
Researcher 31 43%
Student > Ph. D. Student 12 17%
Student > Bachelor 6 8%
Other 4 6%
Student > Master 4 6%
Other 8 11%
Unknown 7 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 29 40%
Biochemistry, Genetics and Molecular Biology 13 18%
Medicine and Dentistry 13 18%
Engineering 4 6%
Mathematics 2 3%
Other 6 8%
Unknown 5 7%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 January 2022.
All research outputs
#2,474,259
of 22,896,955 outputs
Outputs from BMC Medical Genomics
#88
of 1,225 outputs
Outputs of similar age
#8,018
of 94,176 outputs
Outputs of similar age from BMC Medical Genomics
#2
of 7 outputs
Altmetric has tracked 22,896,955 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,225 research outputs from this source. They receive a mean Attention Score of 4.7. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 94,176 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.