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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Multiplexed measurements of gene signatures in different analytes using the Nanostring nCounter™ Assay System
|
|---|---|
| Published in |
BMC Research Notes, May 2009
|
| DOI | 10.1186/1756-0500-2-80 |
| Pubmed ID | |
| Authors |
Vladislav A Malkov, Kyle A Serikawa, Noel Balantac, James Watters, Gary Geiss, Afshin Mashadi-Hossein, Thomas Fare |
| Abstract |
We assessed NanoString's nCounter Analysis System for its ability to quantify gene expression of forty-eight genes in a single reaction with 100 ng of total RNA or an equivalent amount of tissue lysate. In the nCounter System, multiplexed gene expression target levels are directly detected, without enzymatic reactions, via two sequence-specific probes. The individual mRNA is captured with one mRNA target sequence-specific capture probe that is used in a post-hybridization affinity purification procedure. The second mRNA target specific-sequence and fluorescent-labeled colored coded probe is then used in the detection with the 3-component complex separated on a surface via an applied electric field followed by imaging. We evaluated reproducibility, accuracy, concordance with quantitative RT-PCR, linearity, dynamic range, and the ability of the system to assay different inputs (matched samples of total RNA from Flash Frozen (FF) and Formalin Fixed Paraffin Embedded Tissues (FFPET), and crude tissue lysates (CTL)). |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 141 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 3% |
| Malaysia | 1 | <1% |
| Italy | 1 | <1% |
| Brazil | 1 | <1% |
| Germany | 1 | <1% |
| United Kingdom | 1 | <1% |
| Finland | 1 | <1% |
| Japan | 1 | <1% |
| Spain | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 129 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 41 | 29% |
| Student > Ph. D. Student | 32 | 23% |
| Student > Master | 13 | 9% |
| Professor > Associate Professor | 12 | 9% |
| Other | 9 | 6% |
| Other | 20 | 14% |
| Unknown | 14 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 57 | 40% |
| Biochemistry, Genetics and Molecular Biology | 23 | 16% |
| Medicine and Dentistry | 15 | 11% |
| Immunology and Microbiology | 8 | 6% |
| Engineering | 5 | 4% |
| Other | 15 | 11% |
| Unknown | 18 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 June 2023.
All research outputs
#3,622,393
of 25,374,917 outputs
Outputs from BMC Research Notes
#509
of 4,513 outputs
Outputs of similar age
#12,451
of 103,620 outputs
Outputs of similar age from BMC Research Notes
#3
of 16 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,513 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 103,620 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.