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A leukocyte activation test identifies food items which induce release of DNA by innate immune peripheral blood leucocytes

Overview of attention for article published in Nutrition & Metabolism, April 2018
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (95th percentile)

Mentioned by

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7 news outlets
twitter
6 tweeters
googleplus
1 Google+ user

Citations

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1 Dimensions

Readers on

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16 Mendeley
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Title
A leukocyte activation test identifies food items which induce release of DNA by innate immune peripheral blood leucocytes
Published in
Nutrition & Metabolism, April 2018
DOI 10.1186/s12986-018-0260-4
Pubmed ID
Authors

Irma Garcia-Martinez, Theresa R. Weiss, Muhammad N. Yousaf, Ather Ali, Wajahat Z. Mehal

Abstract

Leukocyte activation (LA) testing identifies food items that induce a patient specific cellular response in the immune system, and has recently been shown in a randomized double blinded prospective study to reduce symptoms in patients with irritable bowel syndrome (IBS). We hypothesized that test reactivity to particular food items, and the systemic immune response initiated by these food items, is due to the release of cellular DNA from blood immune cells. We tested this by quantifying total DNA concentration in the cellular supernatant of immune cells exposed to positive and negative foods from 20 healthy volunteers. To establish if the DNA release by positive samples is a specific phenomenon, we quantified myeloperoxidase (MPO) in cellular supernatants. We further assessed if a particular immune cell population (neutrophils, eosinophils, and basophils) was activated by the positive food items by flow cytometry analysis. To identify the signaling pathways that are required for DNA release we tested if specific inhibitors of key signaling pathways could block DNA release. Foods with a positive LA test result gave a higher supernatant DNA content when compared to foods with a negative result. This was specific as MPO levels were not increased by foods with a positive LA test. Protein kinase C (PKC) inhibitors resulted in inhibition of positive food stimulated DNA release. Positive foods resulted in CD63 levels greater than negative foods in eosinophils in 76.5% of tests. LA test identifies food items that result in release of DNA and activation of peripheral blood innate immune cells in a PKC dependent manner, suggesting that this LA test identifies food items that result in release of inflammatory markers and activation of innate immune cells. This may be the basis for the improvement in symptoms in IBS patients who followed an LA test guided diet.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 19%
Researcher 3 19%
Student > Doctoral Student 3 19%
Other 1 6%
Student > Ph. D. Student 1 6%
Other 3 19%
Unknown 2 13%
Readers by discipline Count As %
Medicine and Dentistry 6 38%
Agricultural and Biological Sciences 2 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Nursing and Health Professions 1 6%
Sports and Recreations 1 6%
Other 2 13%
Unknown 3 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 58. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 April 2018.
All research outputs
#390,863
of 15,917,147 outputs
Outputs from Nutrition & Metabolism
#82
of 775 outputs
Outputs of similar age
#13,541
of 281,885 outputs
Outputs of similar age from Nutrition & Metabolism
#1
of 1 outputs
Altmetric has tracked 15,917,147 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 775 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 21.9. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 281,885 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them