Title |
In vitro nuclear interactome of the HIV-1 Tat protein
|
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Published in |
Retrovirology, May 2009
|
DOI | 10.1186/1742-4690-6-47 |
Pubmed ID | |
Authors |
Virginie W Gautier, Lili Gu, Niaobh O'Donoghue, Stephen Pennington, Noreen Sheehy, William W Hall |
Abstract |
One facet of the complexity underlying the biology of HIV-1 resides not only in its limited number of viral proteins, but in the extensive repertoire of cellular proteins they interact with and their higher-order assembly. HIV-1 encodes the regulatory protein Tat (86-101aa), which is essential for HIV-1 replication and primarily orchestrates HIV-1 provirus transcriptional regulation. Previous studies have demonstrated that Tat function is highly dependent on specific interactions with a range of cellular proteins. However they can only partially account for the intricate molecular mechanisms underlying the dynamics of proviral gene expression. To obtain a comprehensive nuclear interaction map of Tat in T-cells, we have designed a proteomic strategy based on affinity chromatography coupled with mass spectrometry. |
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