Title |
Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca) BAC clones for terpenoid synthase and cytochrome P450 genes involved in conifer defence reveal insights into a conifer genome
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Published in |
BMC Plant Biology, August 2009
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DOI | 10.1186/1471-2229-9-106 |
Pubmed ID | |
Authors |
Björn Hamberger, Dawn Hall, Mack Yuen, Claire Oddy, Britta Hamberger, Christopher I Keeling, Carol Ritland, Kermit Ritland, Jörg Bohlmann |
Abstract |
Conifers are a large group of gymnosperm trees which are separated from the angiosperms by more than 300 million years of independent evolution. Conifer genomes are extremely large and contain considerable amounts of repetitive DNA. Currently, conifer sequence resources exist predominantly as expressed sequence tags (ESTs) and full-length (FL)cDNAs. There is no genome sequence available for a conifer or any other gymnosperm. Conifer defence-related genes often group into large families with closely related members. The goals of this study are to assess the feasibility of targeted isolation and sequence assembly of conifer BAC clones containing specific genes from two large gene families, and to characterize large segments of genomic DNA sequence for the first time from a conifer. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 2% |
Brazil | 1 | 2% |
Sweden | 1 | 2% |
India | 1 | 2% |
Canada | 1 | 2% |
Spain | 1 | 2% |
Japan | 1 | 2% |
Serbia | 1 | 2% |
Unknown | 55 | 87% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 23 | 37% |
Student > Ph. D. Student | 13 | 21% |
Professor | 6 | 10% |
Student > Postgraduate | 6 | 10% |
Student > Master | 6 | 10% |
Other | 4 | 6% |
Unknown | 5 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 42 | 67% |
Biochemistry, Genetics and Molecular Biology | 11 | 17% |
Chemistry | 2 | 3% |
Environmental Science | 1 | 2% |
Medicine and Dentistry | 1 | 2% |
Other | 1 | 2% |
Unknown | 5 | 8% |