Title |
Human plasma enhances the expression of Staphylococcal microbial surface components recognizing adhesive matrix molecules promoting biofilm formation and increases antimicrobial tolerance In Vitro
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Published in |
BMC Research Notes, July 2014
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DOI | 10.1186/1756-0500-7-457 |
Pubmed ID | |
Authors |
Anthony P Cardile, Carlos J Sanchez, Meghan E Samberg, Desiree R Romano, Sharanda K Hardy, Joseph C Wenke, Clinton K Murray, Kevin S Akers |
Abstract |
Microbial biofilms have been associated with the development of chronic human infections and represent a clinical challenge given their increased antimicrobial tolerance. Staphylococcus aureus is a major human pathogen causing a diverse range of diseases, of which biofilms are often involved. Staphylococcal attachment and the formation of biofilms have been shown to be facilitated by host factors that accumulate on surfaces. To better understand how host factors enhance staphylococcal biofilm formation, we evaluated the effect of whole human plasma on biofilm formation in clinical isolates of S. aureus and the expression of seven microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) known to be involved in biofilm formation by quantitative real-time PCR. We also evaluated whether plasma augmented changes in S. aureus biofilm morphology and antimicrobial resistance. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 1% |
Unknown | 95 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 19 | 20% |
Student > Master | 17 | 18% |
Student > Ph. D. Student | 15 | 16% |
Researcher | 11 | 11% |
Student > Doctoral Student | 7 | 7% |
Other | 10 | 10% |
Unknown | 17 | 18% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 21 | 22% |
Agricultural and Biological Sciences | 18 | 19% |
Medicine and Dentistry | 16 | 17% |
Immunology and Microbiology | 14 | 15% |
Veterinary Science and Veterinary Medicine | 2 | 2% |
Other | 6 | 6% |
Unknown | 19 | 20% |