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The effect of carbohydrate moiety structure on the immunoregulatory activity of lactoferrin in vitro

Overview of attention for article published in Cellular & Molecular Biology Letters, May 2014
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  • Good Attention Score compared to outputs of the same age (67th percentile)

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Title
The effect of carbohydrate moiety structure on the immunoregulatory activity of lactoferrin in vitro
Published in
Cellular & Molecular Biology Letters, May 2014
DOI 10.2478/s11658-014-0196-2
Pubmed ID
Authors

Michał Zimecki, Jolanta Artym, Maja Kocięba, Maria Duk, Marian Kruzel

Abstract

The aim of this study was to evaluate the immunoregulatory effects of recombinant human lactoferrin (rhLF) in two in vitro models: (1) the secondary humoral immune response to sheep erythrocytes (SRBC); and (2) the mixed lymphocyte reaction (MLR). We compared the non-sialylated glycoform of rhLF as expressed by glycoengineered Pichia pastoris with one that was further chemically sialylated. In an earlier study, we showed that sialylated rhLF could reverse methotrexate-induced suppression of the secondary immune response of mouse splenocytes to SRBC, and that the phenomenon is dependent on the interaction of lactoferrin (LF) with sialoadhesin (CD169). We found that the immunorestorative activity of sialylated rhLF is also dependent on its interaction with the CD22 antigen, a member of the immunoglobulin superfamily that is expressed by B lymphocytes. We also demonstrated that only sialylated rhLF was able to inhibit the MLR reaction. MLR was inhibited by bovine lactoferrin (bLF), a glycoform that has a more complex glycan structure. Desialylated bLF and lactoferricin, a bLF-derived peptide devoid of carbohydrates, did not express such inhibitory activity. We showed that the interaction of LF with sialic acid receptors is essential for at least some of the immunoregulatory activity of this glycoprotein.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 29%
Student > Ph. D. Student 3 14%
Student > Bachelor 2 10%
Student > Postgraduate 2 10%
Student > Doctoral Student 1 5%
Other 2 10%
Unknown 5 24%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 19%
Biochemistry, Genetics and Molecular Biology 3 14%
Medicine and Dentistry 2 10%
Immunology and Microbiology 2 10%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Other 3 14%
Unknown 6 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 March 2021.
All research outputs
#8,261,756
of 25,373,627 outputs
Outputs from Cellular & Molecular Biology Letters
#62
of 606 outputs
Outputs of similar age
#76,530
of 241,837 outputs
Outputs of similar age from Cellular & Molecular Biology Letters
#1
of 3 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 606 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 241,837 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them