Title |
Analysis of cytomegalovirus-specific T-cell responses in patients with hypertension: comparison of assay methods and antigens
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Published in |
Clinical Hypertension, March 2018
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DOI | 10.1186/s40885-018-0090-8 |
Pubmed ID | |
Authors |
Jong-Chan Youn, Jun Yong Kim, Min Kyung Jung, Hee Tae Yu, Su-Hyung Park, In-Cheol Kim, Sun Ki Lee, Suk-Won Choi, Seongwoo Han, Kyu-Hyung Ryu, Sungha Park, Eui-Cheol Shin |
Abstract |
Recent studies suggest an association between cytomegalovirus (CMV) infection and hypertension. In the present study, we used a variety of antigens and different assay methods to investigate the relationship between CMV-specific T-cell responses and arterial stiffness in patients with hypertension. To evaluate arterial stiffness, pulse wave velocity (PWV) was measured in 207 hypertensive patients (average age, 63 ± 8 years). To measure CMV pp65 and IE-1-specific T-cell responses, we performed intracellular cytokine staining (ICS) and enzyme-linked immunospot (ELISPOT) assays. We also analyzed CMV-specific T-cell responses against 10 different CMV antigens using ELISPOT assays. In patients with hypertension, senescent CD8+ T-cell frequencies were significantly correlated with arterial stiffness. Moreover, arterial stiffness was independently associated with CMV pp65-specific CD8+ T-cell responses as measured by ICS. CMV-specific CD8+ T-cell responses measured by ICS and ELISPOT assays showed good agreement and significant correlation with each other. ELISPOT analyses against 10 different CMV antigens revealed a consistent response pattern irrespective of age, gender, and diabetes. CMV pp65-specific CD8+ T-cell responses were independently correlated with arterial stiffness in patients with hypertension. Additionally, the results of ICS and ELISPOT assays showed a significant correlation and good agreement with each other. These findings are important for guiding choices regarding the broad clinical application of CMV-specific T-cell response assays in this patient population. |
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Members of the public | 1 | 100% |
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Student > Ph. D. Student | 4 | 24% |
Researcher | 3 | 18% |
Student > Bachelor | 2 | 12% |
Student > Master | 2 | 12% |
Lecturer | 1 | 6% |
Other | 1 | 6% |
Unknown | 4 | 24% |
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Biochemistry, Genetics and Molecular Biology | 2 | 12% |
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Materials Science | 1 | 6% |
Other | 0 | 0% |
Unknown | 5 | 29% |