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Analysis of cytomegalovirus-specific T-cell responses in patients with hypertension: comparison of assay methods and antigens

Overview of attention for article published in Clinical Hypertension, March 2018
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Title
Analysis of cytomegalovirus-specific T-cell responses in patients with hypertension: comparison of assay methods and antigens
Published in
Clinical Hypertension, March 2018
DOI 10.1186/s40885-018-0090-8
Pubmed ID
Authors

Jong-Chan Youn, Jun Yong Kim, Min Kyung Jung, Hee Tae Yu, Su-Hyung Park, In-Cheol Kim, Sun Ki Lee, Suk-Won Choi, Seongwoo Han, Kyu-Hyung Ryu, Sungha Park, Eui-Cheol Shin

Abstract

Recent studies suggest an association between cytomegalovirus (CMV) infection and hypertension. In the present study, we used a variety of antigens and different assay methods to investigate the relationship between CMV-specific T-cell responses and arterial stiffness in patients with hypertension. To evaluate arterial stiffness, pulse wave velocity (PWV) was measured in 207 hypertensive patients (average age, 63 ± 8 years). To measure CMV pp65 and IE-1-specific T-cell responses, we performed intracellular cytokine staining (ICS) and enzyme-linked immunospot (ELISPOT) assays. We also analyzed CMV-specific T-cell responses against 10 different CMV antigens using ELISPOT assays. In patients with hypertension, senescent CD8+ T-cell frequencies were significantly correlated with arterial stiffness. Moreover, arterial stiffness was independently associated with CMV pp65-specific CD8+ T-cell responses as measured by ICS. CMV-specific CD8+ T-cell responses measured by ICS and ELISPOT assays showed good agreement and significant correlation with each other. ELISPOT analyses against 10 different CMV antigens revealed a consistent response pattern irrespective of age, gender, and diabetes. CMV pp65-specific CD8+ T-cell responses were independently correlated with arterial stiffness in patients with hypertension. Additionally, the results of ICS and ELISPOT assays showed a significant correlation and good agreement with each other. These findings are important for guiding choices regarding the broad clinical application of CMV-specific T-cell response assays in this patient population.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 24%
Researcher 3 18%
Student > Bachelor 2 12%
Student > Master 2 12%
Lecturer 1 6%
Other 1 6%
Unknown 4 24%
Readers by discipline Count As %
Medicine and Dentistry 5 29%
Immunology and Microbiology 3 18%
Biochemistry, Genetics and Molecular Biology 2 12%
Agricultural and Biological Sciences 1 6%
Materials Science 1 6%
Other 0 0%
Unknown 5 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 April 2018.
All research outputs
#22,767,715
of 25,382,440 outputs
Outputs from Clinical Hypertension
#81
of 98 outputs
Outputs of similar age
#307,617
of 348,083 outputs
Outputs of similar age from Clinical Hypertension
#3
of 3 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 98 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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