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Targeted genome modifications in soybean with CRISPR/Cas9

Overview of attention for article published in BMC Biotechnology, March 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#10 of 883)
  • High Attention Score compared to outputs of the same age (96th percentile)

Mentioned by

news
3 news outlets
blogs
2 blogs
twitter
19 tweeters
patent
8 patents
facebook
3 Facebook pages

Citations

dimensions_citation
308 Dimensions

Readers on

mendeley
491 Mendeley
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Title
Targeted genome modifications in soybean with CRISPR/Cas9
Published in
BMC Biotechnology, March 2015
DOI 10.1186/s12896-015-0131-2
Pubmed ID
Authors

Thomas B Jacobs, Peter R LaFayette, Robert J Schmitz, Wayne A Parrott

Abstract

The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms. Here, the CRISPR/Cas9 system is shown to be effective in soybean by knocking-out a green fluorescent protein (GFP) transgene and modifying nine endogenous loci. Targeted DNA mutations were detected in 95% of 88 hairy-root transgenic events analyzed. Bi-allelic mutations were detected in events transformed with eight of the nine targeting vectors. Small deletions were the most common type of mutation produced, although SNPs and short insertions were also observed. Homoeologous genes were successfully targeted singly and together, demonstrating that CRISPR/Cas9 can both selectively, and generally, target members of gene families. Somatic embryo cultures were also modified to enable the production of plants with heritable mutations, with the frequency of DNA modifications increasing with culture time. A novel cloning strategy and vector system based on In-Fusion® cloning was developed to simplify the production of CRISPR/Cas9 targeting vectors, which should be applicable for targeting any gene in any organism. The CRISPR/Cas9 is a simple, efficient, and highly specific genome editing tool in soybean. Although some vectors are more efficient than others, it is possible to edit duplicated genes relatively easily. The vectors and methods developed here will be useful for the application of CRISPR/Cas9 to soybean and other plant species.

Twitter Demographics

The data shown below were collected from the profiles of 19 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 491 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 <1%
Spain 2 <1%
Belgium 1 <1%
Argentina 1 <1%
Peru 1 <1%
Denmark 1 <1%
China 1 <1%
Italy 1 <1%
Japan 1 <1%
Other 1 <1%
Unknown 477 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 107 22%
Researcher 98 20%
Student > Master 78 16%
Student > Bachelor 56 11%
Student > Doctoral Student 23 5%
Other 60 12%
Unknown 69 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 268 55%
Biochemistry, Genetics and Molecular Biology 118 24%
Environmental Science 4 <1%
Chemistry 3 <1%
Medicine and Dentistry 3 <1%
Other 14 3%
Unknown 81 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 51. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 April 2021.
All research outputs
#562,951
of 19,157,212 outputs
Outputs from BMC Biotechnology
#10
of 883 outputs
Outputs of similar age
#8,486
of 227,751 outputs
Outputs of similar age from BMC Biotechnology
#1
of 1 outputs
Altmetric has tracked 19,157,212 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 883 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.6. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 227,751 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them