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Design and evaluation of a unique SYBR Green real-time RT-PCR assay for quantification of five major cytokines in cattle, sheep and goats

Overview of attention for article published in BMC Veterinary Research, January 2015
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (72nd percentile)

Mentioned by

twitter
3 tweeters
patent
1 patent

Citations

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42 Dimensions

Readers on

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86 Mendeley
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Title
Design and evaluation of a unique SYBR Green real-time RT-PCR assay for quantification of five major cytokines in cattle, sheep and goats
Published in
BMC Veterinary Research, January 2015
DOI 10.1186/s12917-015-0382-0
Pubmed ID
Authors

Carinne Puech, Laurence Dedieu, Isabelle Chantal, Valérie Rodrigues

Abstract

Today, when more than 60% of animal diseases are zoonotic, understanding their origin and development and identifying protective immune responses in ruminants are major challenges. Robust, efficient and cost-effective tools are preconditions to solve these challenges. Cytokines play a key role in the main mechanisms by which the immune system is balanced in response to infectious pathogens. The cytokine balance has thus become the focus of research to characterize immune response in ruminants. Currently, SYBR Green reverse transcriptase quantitative PCR (RT-qPCR) is the most widely method used to investigate cytokine gene expression in ruminants, but the conditions in which the many assays are carried out vary considerably and need to be properly evaluated. Accordingly, the quantification of gene expression by RT-qPCR requires normalization by multiple reference genes. The objective of the present study was thus to develop an RT-qPCR assay to simultaneously quantify the expression of several cytokines and reference genes in three ruminant species. In this paper, we detail each stage of the experimental protocol, check validation parameters and report assay performances, following MIQE guidelines.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 86 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 1%
Denmark 1 1%
Unknown 84 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 19%
Researcher 13 15%
Student > Master 13 15%
Student > Doctoral Student 9 10%
Student > Bachelor 9 10%
Other 11 13%
Unknown 15 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 29 34%
Veterinary Science and Veterinary Medicine 15 17%
Biochemistry, Genetics and Molecular Biology 10 12%
Immunology and Microbiology 7 8%
Medicine and Dentistry 5 6%
Other 5 6%
Unknown 15 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2018.
All research outputs
#4,008,846
of 14,843,716 outputs
Outputs from BMC Veterinary Research
#288
of 2,189 outputs
Outputs of similar age
#60,329
of 221,014 outputs
Outputs of similar age from BMC Veterinary Research
#1
of 1 outputs
Altmetric has tracked 14,843,716 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 2,189 research outputs from this source. They receive a mean Attention Score of 3.0. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 221,014 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them