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Interaction between mesenchymal stem cells and endothelial cells restores endothelial permeability via paracrine hepatocyte growth factor in vitro

Overview of attention for article published in Stem Cell Research & Therapy, March 2015
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2 tweeters

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66 Dimensions

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64 Mendeley
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Title
Interaction between mesenchymal stem cells and endothelial cells restores endothelial permeability via paracrine hepatocyte growth factor in vitro
Published in
Stem Cell Research & Therapy, March 2015
DOI 10.1186/s13287-015-0025-1
Pubmed ID
Authors

Qi-Hong Chen, Ai-Ran Liu, Hai-Bo Qiu, Yi Yang

Abstract

Mesenchymal stem cells (MSCs) have potent stabilising effects on vascular endothelium injury, inhibiting endothelial permeability in lung injury via paracrine hepatocyte growth factor (HGF). Recently, it has been indicated that MSCs secrete more factors by MSC-endothelial cell (MSC-EC) interactions. We hypothesised that MSC-EC interactions restore endothelial permeability induced by lipopolysaccharide (LPS) via paracrine HGF. We investigated the endothelial permeability induced by LPS under two co-culture conditions. Human pulmonary microvascular endothelial cells (HPMECs) were added into the upper chambers of cell-culture inserts, while two different co-culture conditions were used in the lower side of the transwells, as follows: 1) MSC-EC interaction group: MSCs and HPMECs contact co-culture; 2) MSC group: MSCs only. The endothelial paracellular and transcellular permeability in the upper side of transwells were detected. Then, the concentration of HGF was measured in the culture medium using an Enzyme Linked Immunosorbent Assay (ELISA) kit, followed by neutralisation of HGF with anti-HGF antibody in the co-culture medium. In addition, Adherens junction and cytoskeleton proteins expression were measured by Western blot and immunofluorescence. HPMECs proliferation was analyzed by Bromodeoxyuridine incorporation assay. The paracellular permeability significantly increased after LPS stimulation in a dose-dependent and time-dependent manner. Meanwhile, MSC-EC interaction more significantly decreased endothelial paracellular and transcellular permeability induced by LPS. Moreover, HGF levels in the MSC-EC interaction group were much higher than those of the MSC group. However, neutralising HGF with anti-HGF antibody inhibited the role of MSC-EC interaction in improving endothelial permeability. Compared to the MSC group, MSC-EC interaction increased VE-cadherin and occludin protein expression, reduced caveolin-1 protein expression in HPMECs and restored remodelling of F-actin and junctional localisation of vascular endothelial cadherin (VE)-cadherin. Furthermore proliferation ratio in MSC-EC interaction group was higher than that of MSC group. However, the effects of MSCs was significantly blocked by anti-HGF antibody. These data suggested that MSC-EC interaction decreased endothelial permeability induced by LPS, which was mainly attributed to HGF secreted by MSCs. The main mechanisms by which HGF restored the integrity of endothelial monolayers was remodelling of endothelial intercellular junctions, decreasing caveolin-1 protein expression and inducing proliferation in HPMECs.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 2%
Germany 1 2%
Brazil 1 2%
Unknown 61 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 28%
Student > Master 11 17%
Researcher 9 14%
Student > Bachelor 8 13%
Student > Doctoral Student 4 6%
Other 5 8%
Unknown 9 14%
Readers by discipline Count As %
Medicine and Dentistry 15 23%
Agricultural and Biological Sciences 13 20%
Biochemistry, Genetics and Molecular Biology 11 17%
Engineering 5 8%
Chemistry 2 3%
Other 8 13%
Unknown 10 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2015.
All research outputs
#8,463,673
of 14,629,909 outputs
Outputs from Stem Cell Research & Therapy
#628
of 1,354 outputs
Outputs of similar age
#103,448
of 222,401 outputs
Outputs of similar age from Stem Cell Research & Therapy
#5
of 7 outputs
Altmetric has tracked 14,629,909 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,354 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has gotten more attention than average, scoring higher than 50% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 222,401 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.