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Reelin expression in brain endothelial cells: an electron microscopy study

Overview of attention for article published in BMC Neuroscience, March 2015
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Title
Reelin expression in brain endothelial cells: an electron microscopy study
Published in
BMC Neuroscience, March 2015
DOI 10.1186/s12868-015-0156-4
Pubmed ID
Authors

Emma Perez-Costas, Erin Y Fenton, Hector J Caruncho

Abstract

Reelin expression and function have been extensively studied in the brain, although its expression has been also reported in other tissues including blood. This raises the possibility that reelin might be able to cross the blood-brain barrier, which could be functionally relevant. Up-to-date no studies have been conducted to assess if reelin is present in the blood-brain barrier, which is mainly constituted by tightly packed endothelial cells. In this report we assessed the expression of reelin in brain capillaries using immunocytochemistry and electron microscopy. At the light microscope, reelin immunolabeling appeared in specific endothelial cells in brain areas that presented abundant diffuse labeling for this protein (e.g., layer I of the cortex, or the stratum lacunosum moleculare of the hippocampus), while it was mostly absent from capillaries in other brain areas (e.g., deeper cortical layers, or the CA1 layer of the hippocampus). As expected, at the electron microscope reelin labeling was observed in neurons of the cortex, where most of the labeling was associated with the rough endoplasmic reticulum. Importantly, reelin was also observed in some endothelial cells located in small capillaries, which confirmed the findings obtained at the light microscope. In these cells, reelin labeling was located primarily in caveolae (i.e., vesicles of transcytosis), and associated with the plasma membrane of the luminal side of endothelial cells. In addition, some scarce labeling was observed in the nuclear membrane. The presence of reelin immunolabeling in brain endothelial cells, and particularly in caveolar vesicles within these cells, suggests that reelin and/or reelin peptides may be able to cross the blood-brain barrier, which could have important physiological, pathological, and therapeutic implications.

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Mendeley readers

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The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
Brazil 1 3%
Unknown 29 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 32%
Student > Master 6 19%
Researcher 3 10%
Student > Postgraduate 2 6%
Professor > Associate Professor 2 6%
Other 3 10%
Unknown 5 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 19%
Biochemistry, Genetics and Molecular Biology 5 16%
Medicine and Dentistry 5 16%
Neuroscience 5 16%
Engineering 2 6%
Other 1 3%
Unknown 7 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 August 2021.
All research outputs
#18,403,994
of 22,796,179 outputs
Outputs from BMC Neuroscience
#881
of 1,244 outputs
Outputs of similar age
#192,594
of 263,362 outputs
Outputs of similar age from BMC Neuroscience
#20
of 24 outputs
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