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Genome sequencing of Clostridium butyricum DKU-01, isolated from infant feces

Overview of attention for article published in Gut Pathogens, March 2015
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (86th percentile)
  • High Attention Score compared to outputs of the same age and source (87th percentile)

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7 X users

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Title
Genome sequencing of Clostridium butyricum DKU-01, isolated from infant feces
Published in
Gut Pathogens, March 2015
DOI 10.1186/s13099-015-0055-3
Pubmed ID
Authors

SangJoon Mo, Bong-Soo Kim, Sung-Jo Yun, Jung-Ju Lee, Suk-Hyun Yoon, Chung-Hun Oh

Abstract

Clostridium butyricum is a butyric acid-producing anaerobic bacteriuma, and commonly present as gut microbiota in humans. This species has been used as a probiotic for the prevention of diarrhea in humans. In this study, we report the draft genome of C. butyricum DKU-01, which was isolated from infant feces, to better understand the characteristics of this strain so that it can later be used in the development of probiotic products. A total of 79 contigs generated by hybrid assembly of sequences obtained from Roche 454 and Illumina Miseq sequencing systems were investigated. The assembled genome of strain DKU-01 consisted of 4,519,722 bp (28.62% G + C content) with a N50 contig length of 108,221 bp and 4,037 predicted CDSs. The extracted 16S rRNA gene from genome sequences of DKU-01 was similar to Clostridium butyricum with 99.63% pairwise similarity. The sequence of strain DKU-01 was compared with previously reported genome sequences of C. butyricum. The value of average nucleotide identity between strains DKU-01 and C. butyricum 60E3 was 98.74%, making it the most similar strain to DKU-01. We sequenced the DKU-01 strain isolated from infant feces, and compared it with the available genomes of C. butyricum on a public database. Genes related to Fructooligosaccharide utilization were detected in the genome of strain DKU-01 and compared with other genera, such as Bifidobacterium and Streptococcus. We found that strain DKU-01 can metabolize a wide range of carbohydrates in comparative genome result. Further analyses of the comparative genome and fermentation study can provide the information necessary for the development of strain DKU-01 for probiotics.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 41 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 22%
Student > Master 8 20%
Researcher 6 15%
Other 3 7%
Student > Postgraduate 3 7%
Other 4 10%
Unknown 8 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 20%
Agricultural and Biological Sciences 8 20%
Medicine and Dentistry 5 12%
Immunology and Microbiology 3 7%
Nursing and Health Professions 2 5%
Other 6 15%
Unknown 9 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2015.
All research outputs
#2,649,862
of 22,796,179 outputs
Outputs from Gut Pathogens
#64
of 521 outputs
Outputs of similar age
#35,823
of 263,549 outputs
Outputs of similar age from Gut Pathogens
#1
of 8 outputs
Altmetric has tracked 22,796,179 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 521 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.8. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,549 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them