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Proficiency testing for bacterial whole genome sequencing: an end-user survey of current capabilities, requirements and priorities

Overview of attention for article published in BMC Infectious Diseases, April 2015
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Title
Proficiency testing for bacterial whole genome sequencing: an end-user survey of current capabilities, requirements and priorities
Published in
BMC Infectious Diseases, April 2015
DOI 10.1186/s12879-015-0902-3
Pubmed ID
Authors

Jacob Moran-Gilad, Vitali Sintchenko, Susanne Karlsmose Pedersen, William J Wolfgang, James Pettengill, Errol Strain, Rene S Hendriksen, on behalf of the Global Microbial Identifier initiative’s Working Group 4 (GMI-WG4)

Abstract

The advent of next-generation sequencing (NGS) has revolutionised public health microbiology. Given the potential impact of NGS, it is paramount to ensure standardisation of 'wet' laboratory and bioinformatic protocols and promote comparability of methods employed by different laboratories and their outputs. Therefore, one of the ambitious goals of the Global Microbial Identifier (GMI) initiative ( http://www.globalmicrobialidentifier.org/ ) has been to establish a mechanism for inter-laboratory NGS proficiency testing (PT). This report presents findings from the survey recently conducted by Working Group 4 among GMI members in order to ascertain NGS end-use requirements and attitudes towards NGS PT. The survey identified the high professional diversity of laboratories engaged in NGS-based public health projects and the wide range of capabilities within institutions, at a notable range of costs. The priority pathogens reported by respondents reflected the key drivers for NGS use (high burden disease and 'high profile' pathogens). The performance of and participation in PT was perceived as important by most respondents. The wide range of sequencing and bioinformatics practices reported by end-users highlights the importance of standardisation and harmonisation of NGS in public health and underpins the use of PT as a means to assuring quality. The findings of this survey will guide the design of the GMI PT program in relation to the spectrum of pathogens included, testing frequency and volume as well as technical requirements. The PT program for external quality assurance will evolve and inform the introduction of NGS into clinical and public health microbiology practice in the post-genomic era.

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The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 97 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Sweden 1 1%
Denmark 1 1%
Canada 1 1%
Unknown 94 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 24 25%
Student > Ph. D. Student 15 15%
Student > Master 14 14%
Student > Bachelor 6 6%
Student > Doctoral Student 5 5%
Other 14 14%
Unknown 19 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 29 30%
Biochemistry, Genetics and Molecular Biology 14 14%
Medicine and Dentistry 8 8%
Immunology and Microbiology 8 8%
Business, Management and Accounting 3 3%
Other 15 15%
Unknown 20 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 May 2015.
All research outputs
#14,220,809
of 22,797,621 outputs
Outputs from BMC Infectious Diseases
#3,773
of 7,674 outputs
Outputs of similar age
#139,543
of 264,242 outputs
Outputs of similar age from BMC Infectious Diseases
#61
of 138 outputs
Altmetric has tracked 22,797,621 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,674 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.6. This one is in the 47th percentile – i.e., 47% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,242 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 138 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.